Cloned (Comment) | Organism |
---|---|
expression of MDH in Escherichia coli strain BL21(DE3) in inclusion bodies | Aeropyrum pernix |
Crystallization (Comment) | Organism |
---|---|
purified refolded reconbinant enzyme, sitting drop vapor diffusion method, 0.002 ml of protein solution containing 8.9 mg/ml protein in 50 mM Tris-HCl, pH7.5, containing 0.2 M NaCl, are mixed with an equal volume of the reservoir solution, comprised of 0.1 M CHES, pH 9.5, and 40% PEG 600, and equilibrated against 0.15 ml of reservoir solution at 20°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.9 A resolution and room temperature | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
(S)-malate | pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme | Aeropyrum pernix | |
0.12 | - |
(S)-malate | pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme | Aeropyrum pernix | |
0.2 | - |
(2S,3R)-tartrate | pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme | Aeropyrum pernix | |
1.2 | - |
(2S,3S)-tartrate | pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme | Aeropyrum pernix | |
2 | 3 | (2S,3S)-tartrate | pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme | Aeropyrum pernix | |
5.8 | - |
(2S,3R)-tartrate | pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33489 | - |
4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation | Aeropyrum pernix |
34000 | - |
4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation | Aeropyrum pernix |
110000 | - |
recombinant enzyme, gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YEA1 | a strict aerobic hyperthermophilic archaeon isolated from a coastal thermal vent in Japan | - |
Purification (Comment) | Organism |
---|---|
recombinant MDH from Escherichia coli inclusion bodies by solubilization, refolding, dialysis, and gel filtration | Aeropyrum pernix |
Renatured (Comment) | Organism |
---|---|
solubilization and refolding of recombinant MDH from Escherichia coli inclusion bodies, the enzyme is dissolved in 6 M guanidine-HCl and gradually refolded to the active enzyme through dilution of the denaturant, conditions: refolding buffer 1.5 L of 0.1 M Tris-HCl, pH 7.5, containing 2 mM EDTA and 0.4 M L-arginine, incubation for 36 h at 4°C | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R)-tartrate + NAD(P)+ | - |
Aeropyrum pernix | ? + NAD(P)H + H+ | - |
? | |
(2S,3S)-tartrate + NAD(P)+ | - |
Aeropyrum pernix | ? + NAD(P)H + H+ | - |
? | |
(S)-malate + NAD(P)+ | - |
Aeropyrum pernix | oxaloacetate + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure qand sequence comaprsion, overall structure, overview | Aeropyrum pernix |
tetramer | 4 * 34000, recombinant enzyme, SDS-PAGE, 4 * 33489, sequence calculation | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
(S)-malate dehydrogenase | - |
Aeropyrum pernix |
MDH | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
above | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 95 | - |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, 30 min, pH 5.0-10.5, stable | Aeropyrum pernix |
90 | - |
purified recombinant enzyme, 10 min, stable. The hyperthermostability of the Aeropyrum pernix MDH is likely attributable to its smaller cavity volume and larger numbers of ion pairs and ion-pair networks, but the molecular strategy for thermostability may be specific for each enzyme | Aeropyrum pernix |
100 | - |
purified recombinant enzyme, 10 min, stable. The hyperthermostability of the Aeropyrum pernix MDH is likely attributable to its smaller cavity volume and larger numbers of ion pairs and ion-pair networks, but the molecular strategy for thermostability may be specific for each enzyme | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
(2S,3R)-tartrate | pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme | Aeropyrum pernix | |
0.2 | - |
(2S,3R)-tartrate | pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme | Aeropyrum pernix | |
0.37 | - |
(2S,3S)-tartrate | pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme | Aeropyrum pernix | |
1.4 | - |
(S)-malate | pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme | Aeropyrum pernix | |
2.6 | - |
(S)-malate | pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme | Aeropyrum pernix | |
4.1 | - |
(2S,3S)-tartrate | pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Aeropyrum pernix |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | purified recombinant enzyme, 30 min, 50°C, stable | Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ is the preferred cofactor compared to NAD+ with substrate (S)-malate, while NAD+ is preferred with substrate tartrate. Ala at position 53 is responsible for coenzyme specificity, and the next residue, Arg, is important for NADP+ binding, structural analysis and comparison | Aeropyrum pernix | |
NADP+ | NADP+ is the preferred cofactor compared to NAD+ with substrate (S)-malate, while NAD+ is preferred with substrate tartrate. Ala at position 53 is responsible for coenzyme specificity, and the next residue, Arg, is important for NADP+ binding, structural analysis and comparison | Aeropyrum pernix |