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Literature summary for 1.1.1.85 extracted from

  • Parsons, S.J.; Burns, R.O.
    beta-Isopropylmalate dehydrogenase (Salmonella typhimurium) (1970), Methods Enzymol., 17A, 793-799.
No PubMed abstract available

General Stability

General Stability Organism
dilute solutions, 1 mg/ml or less, precipitate upon thawing Salmonella enterica subsp. enterica serovar Typhimurium
slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Ca2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Co2+ above 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetamide 50 mM, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetate 50 mM, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
K+ above 1 M, slight inhibition Salmonella enterica subsp. enterica serovar Typhimurium
Li+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+ above 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
N-ethylmaleimide
-
Salmonella enterica subsp. enterica serovar Typhimurium
NEM 5 mM, 50% inhibition, not reversed by 2-mercaptoethanol Salmonella enterica subsp. enterica serovar Typhimurium
p-hydroxymercuribenzoate pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol Salmonella enterica subsp. enterica serovar Typhimurium
PCMB
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
(2R,3S)-3-isopropylmalate pH 9.0 Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
NAD+ pH 9.0 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ Salmonella enterica subsp. enterica serovar Typhimurium
K+ monovalent cation required, maximal activity at 0.05 M Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+ divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ Salmonella enterica subsp. enterica serovar Typhimurium
NH4+ monovalent cations required, NH4+ can partially replace K+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl Salmonella enterica subsp. enterica serovar Typhimurium
70000
-
equilibrium sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Salmonella enterica subsp. enterica serovar Typhimurium penultimate step in leucine biosynthesis 2-oxoisocaproate + NADH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
strain leu A-124
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
-20°C, pH 5.5-7.5, fully active Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+ penultimate step in leucine biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
r
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisohexanoate + thionicotinamideNADH + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 stable if stored concentrated Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium