KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
3-ethylmalate | - |
Acidithiobacillus ferrooxidans | |
0.015 | - |
(2R,3S)-3-isopropylmalate | - |
Acidithiobacillus ferrooxidans | |
0.05 | - |
3-methylmalate | - |
Acidithiobacillus ferrooxidans | |
0.074 | - |
3-tert-butylmalate | - |
Acidithiobacillus ferrooxidans | |
0.11 | - |
3-isoamylmalate | - |
Acidithiobacillus ferrooxidans | |
7.7 | - |
malate | - |
Acidithiobacillus ferrooxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Acidithiobacillus ferrooxidans | key enzyme in leucine biosynthesis | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | key enzyme in leucine biosynthesis | Acidithiobacillus ferrooxidans | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
r | |
3-ethylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 3-ethylpyruvate + NADH + CO2 | - |
? | |
3-isoamylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 3-isoamylpyruvate + NADH + CO2 | - |
? | |
3-methylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 3-methylpyruvate + NADH + CO2 | - |
? | |
3-tert-butylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 3-tert-butylpyruvate + NADH + CO2 | - |
? | |
malate + NAD+ | - |
Acidithiobacillus ferrooxidans | pyruvate + NADH + H+ + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
3-tert-butylmalate | - |
Acidithiobacillus ferrooxidans | |
5 | - |
malate | - |
Acidithiobacillus ferrooxidans | |
20 | - |
3-isoamylmalate | - |
Acidithiobacillus ferrooxidans | |
28 | - |
(2R,3S)-3-isopropylmalate | - |
Acidithiobacillus ferrooxidans | |
28 | - |
3-methylmalate | - |
Acidithiobacillus ferrooxidans | |
38 | - |
3-ethylmalate | - |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acidithiobacillus ferrooxidans |