Crystallization (Comment) | Organism |
---|---|
to 2.25 A resolution. Modeling of 3-isopropylmalate binding in the isoform IPMDH2 active site. Results suggest that substitution of one active site residue may lead to altered substrate specificity and metabolic function | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
F137L | mutation to corresponding residue of isoform IMPDH2, reduces activity toward 3-(2'-methylthio)-ethylmalate by 200fold, but enhances catalytic efficiency with 3-isopropylmalate to levels observed with isoform IPMDH2 | Arabidopsis thaliana |
L133F | mutation to corresponding residue of isoform IMPDH1, enhances catalytic efficiency with 3-(2'-methylthio)ethylmalate about100fold and reduces activity for 3-isopropylmalate | Arabidopsis thaliana |
L134F | mutation to corresponding residue of isoform IMPDH1, reduces activity toward 3-(2'-methylthio)-ethylmalate, but enhances catalytic efficiency with 3-isopropylmalate | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0092 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.0109 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.011 | - |
(2R,3S)-3-isopropylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.025 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.028 | - |
(2R,3S)-3-isopropylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.0303 | - |
(2R,3S)-3-isopropylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.045 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.077 | - |
3-(2'-methylthio)ethylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.103 | - |
3-(2'-methylthio)ethylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.323 | - |
3-(2'-methylthio)ethylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.436 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.502 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P93832 | isoform IPMDH2 | - |
Arabidopsis thaliana | Q9FMT1 | isoform IPMDH3 | - |
Arabidopsis thaliana | Q9SA14 | isoform IPMDH1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Arabidopsis thaliana | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
3-(2'-methylthio)ethylmalate + NAD+ | - |
Arabidopsis thaliana | ? + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.0167 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.033 | - |
3-(2'-methylthio)ethylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.27 | - |
3-(2'-methylthio)ethylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.367 | - |
3-(2'-methylthio)ethylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.62 | - |
(2R,3S)-3-isopropylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.622 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.73 | - |
(2R,3S)-3-isopropylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.85 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
3.83 | - |
(2R,3S)-3-isopropylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
6.22 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
9.05 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.1 | - |
3-(2'-methylthio)ethylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.33 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
2.59 | - |
3-(2'-methylthio)ethylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
4.76 | - |
3-(2'-methylthio)ethylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
18.77 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
20.3 | - |
(2R,3S)-3-isopropylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
24.47 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
25.7 | - |
(2R,3S)-3-isopropylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
336.3 | - |
(2R,3S)-3-isopropylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
570 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
983.7 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |