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Literature summary for 1.1.1.86 extracted from

  • Satyanarayana, T.; Radhakrishnan, A.N.
    Biosynthesis of valine and isoleucine in plants. 3. Reductoisomerase of Phaseolus radiatus (1965), Biochim. Biophys. Acta, 110, 380-388.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid 5 mM, enhances activity 2-hydroxy-2-ethyl-3-oxobutanoate as substrate Vigna radiata var. radiata

General Stability

General Stability Organism
high activity loss during DEAE-cellulose chromatography, stable towards acetone fractionation and dialysis, unstable towards freezing and thawing Vigna radiata var. radiata

Inhibitors

Inhibitors Comment Organism Structure
arsenite slight inhibition Vigna radiata var. radiata
ascorbic acid slight inhibition with 2-acetolactate as substrate Vigna radiata var. radiata
L-ascorbic acid slightly inhibitory with alpha-acetolactate as substrate Vigna radiata var. radiata
additional information no inhibition with p-mercuribenzoate, cysteine, 2-mercaptoethanol Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
2-aceto-2-hydroxybutyrate
-
Vigna radiata var. radiata
5.5
-
2-acetolactate
-
Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ions required Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-acetolactate + NADPH Vigna radiata var. radiata enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Vigna radiata var. radiata 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
NADPH + 2-aceto-2-hydroxybutyrate
-
Vigna radiata var. radiata NADP+ + ?
-
?
NADPH + 2-acetolactate
-
Vigna radiata var. radiata NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 constant activity with 2-acetolactate as substrate Vigna radiata var. radiata
8.6
-
with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer, alpha-aceto-alpha-hydroxybutyrate as substrate, Tris buffer Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 40% of maximal activity, pH 9.0: about 90% of maximal activity, with 2-hydroxy-2-ethyl-3-oxobutanoate as substrate, Tris-HCl buffer Vigna radiata var. radiata

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Vigna radiata var. radiata