Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-dimethylphosphinoyl-2-hydroxyacetic acid | nearly irreversible; reversible, strong competitive inhibition | Ipomoea purpurea | |
2-dimethylphosphinoyl-2-hydroxyacetic acid | nearly irreversible; reversible, strong competitive inhibition | Solanum nigrum | |
2-dimethylphosphinoyl-2-hydroxyacetic acid | nearly irreversible; reversible, strong competitive inhibition | Spinacia oleracea | |
N-Hydroxy-N-isopropyloxamate | nearly irreversible, competitive; nearly irreversible, poor herbicidal action | Ipomoea purpurea | |
N-Hydroxy-N-isopropyloxamate | nearly irreversible, competitive; nearly irreversible, poor herbicidal action | Solanum nigrum | |
N-Hydroxy-N-isopropyloxamate | nearly irreversible, competitive; nearly irreversible, poor herbicidal action | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Spinacia oleracea | |
Mg2+ | required | Ipomoea purpurea | |
Mg2+ | required | Solanum nigrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | Spinacia oleracea | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | Ipomoea purpurea | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | Solanum nigrum | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea purpurea | - |
morning glory | - |
Solanum nigrum | - |
black nightshade | - |
Spinacia oleracea | - |
overexpressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Spinacia oleracea | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Ipomoea purpurea | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Solanum nigrum | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
NADPH + 2-acetolactate | - |
Spinacia oleracea | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
NADPH + 2-acetolactate | - |
Ipomoea purpurea | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
NADPH + 2-acetolactate | - |
Solanum nigrum | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Spinacia oleracea | |
NADPH | - |
Ipomoea purpurea | |
NADPH | - |
Solanum nigrum |