Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 5.0, 20% (w/v) w/v polyethylene glycol 1500 | uncultured archaeon |
hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 6.0, 22% (w/v) polyethylene glycol monomethylether 5000 | Ignisphaera aggregans |
hanging drop vapor diffusion method, using 0.25 M NaCl, 28% (w/v) polyethylene glycol 3350 and 0.1 M bis-Tris, pH 5.5 | Azotobacter vinelandii |
hanging drop vapor diffusion method, using 1 M sodium potassium tartrate, 200 mM sodium chloride, 100 mM imidazole pH 8.0 | Alicyclobacillus acidocaldarius subsp. acidocaldarius |
in complex with NADPH and the transition state analogue N-isopropyloxamate and apo-form. The enzyme has a seven-residue specificity loop | Ignisphaera aggregans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Ignisphaera aggregans | |
Mg2+ | required | Azotobacter vinelandii | |
Mg2+ | required | Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
Mg2+ | required | uncultured archaeon |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADH + H+ | Ignisphaera aggregans | - |
(R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | Azotobacter vinelandii | - |
(R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | Alicyclobacillus acidocaldarius subsp. acidocaldarius | - |
(R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | uncultured archaeon | - |
(R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | Ignisphaera aggregans DSM 17230 | - |
(R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | Azotobacter vinelandii ATCC BAA-1303 | - |
(R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | Ignisphaera aggregans | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | Azotobacter vinelandii | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | Alicyclobacillus acidocaldarius subsp. acidocaldarius | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | uncultured archaeon | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | Ignisphaera aggregans DSM 17230 | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | Azotobacter vinelandii ATCC BAA-1303 | - |
(R)-2,3-dihydroxyisovalerate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius subsp. acidocaldarius | C8WR67 | - |
- |
Azotobacter vinelandii | C1DFH7 | - |
- |
Azotobacter vinelandii ATCC BAA-1303 | C1DFH7 | - |
- |
Ignisphaera aggregans | E0SRA9 | - |
- |
Ignisphaera aggregans DSM 17230 | E0SRA9 | - |
- |
uncultured archaeon | Q64BR7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADH + H+ | - |
Ignisphaera aggregans | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | - |
Azotobacter vinelandii | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | - |
uncultured archaeon | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | - |
Ignisphaera aggregans DSM 17230 | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | - |
Azotobacter vinelandii ATCC BAA-1303 | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Ignisphaera aggregans | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Azotobacter vinelandii | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
uncultured archaeon | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Ignisphaera aggregans DSM 17230 | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Azotobacter vinelandii ATCC BAA-1303 | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
acetohydroxyacid isomeroreductase | - |
Ignisphaera aggregans |
acetohydroxyacid isomeroreductase | - |
Azotobacter vinelandii |
acetohydroxyacid isomeroreductase | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius |
acetohydroxyacid isomeroreductase | - |
uncultured archaeon |
AHAIR | - |
Ignisphaera aggregans |
AHAIR | - |
Azotobacter vinelandii |
AHAIR | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius |
AHAIR | - |
uncultured archaeon |
AHIR | - |
Ignisphaera aggregans |
AHIR | - |
Azotobacter vinelandii |
AHIR | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius |
AHIR | - |
uncultured archaeon |
ilvC | - |
Ignisphaera aggregans |
KARI | - |
Ignisphaera aggregans |
KARI | - |
Azotobacter vinelandii |
KARI | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius |
KARI | - |
uncultured archaeon |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-maximal residual activity at 95°C | Ignisphaera aggregans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
the enzyme is highly thermostable with half-maximal residual activity at 95°C | Ignisphaera aggregans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Ignisphaera aggregans | |
NADH | - |
Azotobacter vinelandii | |
NADH | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
NADH | - |
uncultured archaeon | |
NADPH | - |
Ignisphaera aggregans | |
NADPH | - |
Azotobacter vinelandii | |
NADPH | - |
Alicyclobacillus acidocaldarius subsp. acidocaldarius | |
NADPH | - |
uncultured archaeon |