Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Meiothermus ruber |
Protein Variants | Comment | Organism |
---|---|---|
T84S | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Meiothermus ruber |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | wild type enzyme, at pH 7.0 and 50°C | Meiothermus ruber | |
0.04 | - |
(S)-2-acetolactate | mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
0.08 | - |
(S)-2-acetolactate | wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
0.15 | - |
(S)-2-acetolactate | mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
0.24 | - |
(S)-2-acetolactate | wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
- |
Meiothermus ruber |
40000 | - |
1 * 40000, SDS-PAGE | Meiothermus ruber |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meiothermus ruber | D3PT81 | - |
- |
Meiothermus ruber DSM 1279 | D3PT81 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Meiothermus ruber |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADH + H+ | - |
Meiothermus ruber | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADH + H+ | - |
Meiothermus ruber DSM 1279 | (R)-2,3-dihydroxyisovalerate + NAD+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Meiothermus ruber | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r | |
(S)-2-acetolactate + NADPH + H+ | - |
Meiothermus ruber DSM 1279 | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Meiothermus ruber |
monomer | 1 * 39000, calculated from amino acid sequence | Meiothermus ruber |
Synonyms | Comment | Organism |
---|---|---|
KARI | - |
Meiothermus ruber |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Meiothermus ruber |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at 50°C, the enzyme shows an half-life of 71 h | Meiothermus ruber |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
(S)-2-acetolactate | wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
0.98 | - |
NADPH | wild type enzyme, at pH 7.0 and 50°C | Meiothermus ruber | |
1.09 | - |
(S)-2-acetolactate | wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
1.3 | - |
(S)-2-acetolactate | mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
3.81 | - |
(S)-2-acetolactate | mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Meiothermus ruber |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | prefers NADPH over NADH | Meiothermus ruber | |
NADPH | prefers NADPH over NADH | Meiothermus ruber |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
(S)-2-acetolactate | wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
10.2 | - |
(S)-2-acetolactate | wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
25.9 | - |
(S)-2-acetolactate | mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
34.1 | - |
(S)-2-acetolactate | mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C | Meiothermus ruber | |
54.5 | - |
NADPH | wild type enzyme, at pH 7.0 and 50°C | Meiothermus ruber |