Any feedback?
Literature summary for 1.1.1.87 extracted from
- Wild-type and mutant forms of homoisocitric dehydrogenase in the yeast Saccharomycopsis lipolytica (1982), Eur. J. Biochem., 128, 489-494.
General Stability
| General Stability | Organism |
|---|---|
| Mg2+ stabilizes | Saccharomycopsis lipolytica |
| Mn2+ stabilizes | Saccharomycopsis lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoadipate | - |
Saccharomycopsis lipolytica |  |
| homoisocitrate | - |
Saccharomycopsis lipolytica | |
| Oxaloglutarate | - |
Saccharomycopsis lipolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required for optimal activity | Saccharomycopsis lipolytica | |
| Mg2+ | required | Saccharomycopsis lipolytica | |
| Mn2+ | stimulation of oxidative decarboxylation | Saccharomycopsis lipolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
native or denaturing PAGE | Saccharomycopsis lipolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | Saccharomycopsis lipolytica | production of 2-oxoadipic acid, a precursor of lysine biosynthesis | 2-oxoadipate + CO2 + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomycopsis lipolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomycopsis lipolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.304 | - |
- |
Saccharomycopsis lipolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable as ammonium sulfate precipitate | Saccharomycopsis lipolytica |
| 0°C, pH 7.2, rapid loss of activity | Saccharomycopsis lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | - |
Saccharomycopsis lipolytica | 2-oxoadipate + CO2 + NADH | - |
? | |
| 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | production of 2-oxoadipic acid, a precursor of lysine biosynthesis | Saccharomycopsis lipolytica | 2-oxoadipate + CO2 + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, at high protein concentration or in presence of substrate the enzyme exists as dimer | Saccharomycopsis lipolytica |
| monomer | 1 * 48000, native or denaturing PAGE | Saccharomycopsis lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Saccharomycopsis lipolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomycopsis lipolytica | |
| NADH | - |
Saccharomycopsis lipolytica | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
