Application | Comment | Organism |
---|---|---|
biotechnology | strain overexpressing enzyme has improved xylitol productivity, production of up to 57g/l xylitol from 225 g/l D-arabitol, via D-xylulose | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | strain overexpressing enzyme has improved xylitol productivity, production of up to 57g/l xylitol from 225 g/l D-arabitol, via D-xylulose | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, 70% inhibition | Gluconobacter oxydans | |
additional information | not inhibitory: MgCl2, ZnCl2, CoCl2, CuCl2 at 1 mM, EDTA at 10 mM | Gluconobacter oxydans | |
NiCl2 | 1 mM, complete inhibition | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27800, deduced from genen sequence, x * 27000, SDS-PAGE | Gluconobacter oxydans |
27800 | - |
x * 27800, deduced from genen sequence, x * 27000, SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
strain ATCC 621 | - |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
113 | - |
substrate D-xylitol, pH 10, 30°C | Gluconobacter oxydans |
220 | - |
substrate D-sorbitol, pH 10, 30°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
D-xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
D-xylulose + NADH + H+ | - |
Gluconobacter oxydans | D-xylitol + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 27800, deduced from genen sequence, x * 27000, SDS-PAGE | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of D-xylulose | Gluconobacter oxydans |
11 | - |
oxidation of xylitol | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gluconobacter oxydans | |
NADH | - |
Gluconobacter oxydans |