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Literature summary for 1.1.1.9 extracted from

  • Qi, X.H.; Zhu, J.F.; Yun, J.H.; Lin, J.; Qi, Y.L.; Guo, Q.; Xu, H.
    Enhanced xylitol production expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell (2016), J. Biosci. Bioeng., 122, 257-262 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xdh, cloning from strain CGMCC 1.49, DNA and amino acid sequence determination and analysis, functional recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21 Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Gluconobacter oxydans
-
D-xylulose + NADH + H+
-
?
xylitol + NAD+ Gluconobacter oxydans CGMCC 1.49
-
D-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans A0A141BGH5
-
-
Gluconobacter oxydans CGMCC 1.49 A0A141BGH5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Gluconobacter oxydans

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
purified native enzyme, substrate xylitol, pH 7.0, 30°C Gluconobacter oxydans
14.2
-
purified recombinant His6-tagged enzyme, substrate D-mannitol, pH 11.0, 30°C Gluconobacter oxydans
14.2
-
purified recombinant His6-tagged enzyme, substrate xylitol, pH 7.0, 30°C Gluconobacter oxydans
87.8
-
purified recombinant His6-tagged enzyme, substrate xylitol, pH 11.0, 30°C Gluconobacter oxydans
135.2
-
purified recombinant His6-tagged enzyme, substrate D-sorbitol, pH 11.0, 30°C Gluconobacter oxydans
151.4
-
purified recombinant His6-tagged enzyme, substrate D-fructose, pH 11.0, 30°C Gluconobacter oxydans
326.3
-
purified recombinant His6-tagged enzyme, substrate D-xylulose, pH 11.0, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NAD+ low activity compared to xylitol Gluconobacter oxydans D-fructose + NADH + H+
-
r
D-mannitol + NAD+ low activity compared to xylitol Gluconobacter oxydans CGMCC 1.49 D-fructose + NADH + H+
-
r
D-sorbitol + NAD+ 51.3% increased activity compared to xylitol Gluconobacter oxydans L-sorbose + NADH + H+
-
?
D-sorbitol + NAD+ 51.3% increased activity compared to xylitol Gluconobacter oxydans CGMCC 1.49 L-sorbose + NADH + H+
-
?
xylitol + NAD+
-
Gluconobacter oxydans D-xylulose + NADH + H+
-
?
xylitol + NAD+
-
Gluconobacter oxydans D-xylulose + NADH + H+
-
r
xylitol + NAD+
-
Gluconobacter oxydans CGMCC 1.49 D-xylulose + NADH + H+
-
?
xylitol + NAD+
-
Gluconobacter oxydans CGMCC 1.49 D-xylulose + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 28000, yes recombinant His6-tagged enzyme, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
XDH
-
Gluconobacter oxydans
xylitol dehydrogenase
-
Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
oxidation of xylitol Gluconobacter oxydans
35
-
reduction of D-xylulose Gluconobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 over 80% of maximal activity within this range for both reaction directions Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
reduction of D-xylulose Gluconobacter oxydans
11
-
oxidation of xylitol Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
4 8 over 60% of maximal activity within this range for oxidation of xylitol, profile overview Gluconobacter oxydans
10 12 over 60% of maximal activity within this range for reduction of D-xylulose, profile overview Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Gluconobacter oxydans

General Information

General Information Comment Organism
evolution XDH has a TGXXGXXG NAD(H)-binding motif and a YXXXK active site motif, and belongs to the short-chain dehydrogenase/ reductase family Gluconobacter oxydans