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Literature summary for 1.1.1.90 extracted from
- Purification and characterization of an NAD+-dependent XylB-like aryl alcohol dehydrogenase identified in Acinetobacter baylyi ADP1 (2012), Appl. Environ. Microbiol., 78, 8743-8752.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Acinetobacter baylyi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | weak inhibition | Acinetobacter baylyi |  |
| N-ethylmaleimide | - |
Acinetobacter baylyi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NADH | pH 7.4, 30°C | Acinetobacter baylyi | |
| 0.28 | - |
benzaldehyde | pH 7.4, 30°C | Acinetobacter baylyi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.01 mM, residual activity: 0.2% | Acinetobacter baylyi | |
| Cu2+ | 0.1 mM, residual activity: 1.3% | Acinetobacter baylyi | |
| Fe2+ | 1 mM, residual activity: 21.7% | Acinetobacter baylyi | |
| Hg+ | 0.001 mM, residual activity: 0.1% | Acinetobacter baylyi | |
| Mg2+ | 1 mM, activity enhanced to 128% | Acinetobacter baylyi | |
| Mn2+ | 1 mM, activity enhanced to 128% | Acinetobacter baylyi | |
| Zn2+ | 1 mM, residual activity: 1.2% | Acinetobacter baylyi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39500 | - |
SDS-PAGE | Acinetobacter baylyi |
| 72600 | - |
gel filtration | Acinetobacter baylyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter baylyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, affinity chromatography | Acinetobacter baylyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anisaldehyde + NADH + H+ | relative activity: 72.2% | Acinetobacter baylyi | anisic alcohol + NAD+ | - |
r | |
| benzaldehyde + NADH + H+ | relative activity: 100% | Acinetobacter baylyi | benzyl alcohol + NAD+ | - |
r | |
| cinnamaldehyde + NADH + H+ | relative activity: 58.7% | Acinetobacter baylyi | cinnamic alcohol + NAD+ | - |
r | |
| cis-11-hexadecenal + NADH + H+ | relative activity: 4.9% | Acinetobacter baylyi | cis-11-hexadecenol + NAD+ | - |
r | |
| citral + NADH + H+ | relative activity: 7.8% | Acinetobacter baylyi | citryl alcohol + NAD+ | - |
r | |
| citronellal + NADH + H+ | relative activity: 21.8% | Acinetobacter baylyi | citronellol + NAD+ | - |
r | |
| coniferyl alcohol + NAD+ | relative activity: 96% | Acinetobacter baylyi | coniferyl aldehyde + NADH + H+ | - |
r | |
| decanal + NADH + H+ | relative activity: 19.7% | Acinetobacter baylyi | decanol + NAD+ | - |
r | |
| dodecanal + NADH + H+ | relative activity: 8.9% | Acinetobacter baylyi | dodecanol + NAD+ | - |
r | |
| geranial + NADH + H+ | - |
Acinetobacter baylyi | geraniol + NAD+ | - |
r | |
| glutaraldehyde + NADH + H+ | relative activity: 0.6% | Acinetobacter baylyi | ? + NAD+ | - |
r | |
| glyoxal + NADH + H+ | relative activity: 0.7% | Acinetobacter baylyi | ? + NAD+ | - |
r | |
| hexanal + NADH + H+ | relative activity: 6.4% | Acinetobacter baylyi | hexanol + NAD+ | - |
r | |
| octanal + NADH + H+ | relative activity: 36.5% | Acinetobacter baylyi | octanol + NAD+ | - |
r | |
| salicyl aldehyde + NADH + H+ | relative activity: 9% | Acinetobacter baylyi | salicyl alcohol + NAD+ | - |
r | |
| vanillin + NADH + H+ | relative activity: 4% | Acinetobacter baylyi | vanillin alcohol + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39500, gel filtration | Acinetobacter baylyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP1 | - |
Acinetobacter baylyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Acinetobacter baylyi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
enzyme reaches 52% of its maximum activity at 15°C | Acinetobacter baylyi |
| 70 | - |
enzyme loses 94% of its activity after 2 min of incubation at 70°C | Acinetobacter baylyi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8920 | - |
NADH | pH 7.4, 30°C | Acinetobacter baylyi | |
| 12940 | - |
benzaldehyde | pH 7.4, 30°C | Acinetobacter baylyi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Acinetobacter baylyi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | disruption mutant shows similar growth compared to wild-type | Acinetobacter baylyi |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 46700 | - |
benzaldehyde | pH 7.4, 30°C | Acinetobacter baylyi | |
| 528000 | - |
NADH | pH 7.4, 30°C | Acinetobacter baylyi | |
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