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Literature summary for 1.1.1.90 extracted from

  • Ying, X.; Wang, Y.; Xiong, B.; Wu, T.; Xie, L.; Yu, M.; Wang, Z.
    Characterization of an allylic/benzyl alcohol dehydrogenase from Yokenella sp. strain WZY002, an organism potentially useful for the synthesis of alpha,beta-unsaturated alcohols from allylic aldehydes and ketones (2014), Appl. Environ. Microbiol., 80, 2399-2409.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Yokenella sp. WZY002

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Yokenella sp. WZY002
Cu2+
-
Yokenella sp. WZY002
Zn2+
-
Yokenella sp. WZY002

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Yokenella sp. WZY002

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36411
-
-
Yokenella sp. WZY002

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide Yokenella sp. WZY002

Organism

Organism UniProt Comment Textmining
Yokenella sp. WZY002 W6CX26
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NADPH + H+ highest reductive activity with benzaldehyde Yokenella sp. WZY002 benzyl alcohol + NADP+
-
r
benzyl alcohol + NADP+
-
Yokenella sp. WZY002 benzaldehyde + NADPH + H+
-
r
crotonaldehyde + NADPH + H+
-
Yokenella sp. WZY002 crotonyl alcohol + NADP+
-
r
crotonyl alcohol + NADP+ highest oxidative activity with crotyl alcohol Yokenella sp. WZY002 crotonaldehyde + NADPH + H+
-
r
additional information no activity with NADH or NAD+ Yokenella sp. WZY002 ?
-
?

Subunits

Subunits Comment Organism
? x * 36411, calculated from amino acid sequence Yokenella sp. WZY002

Synonyms

Synonyms Comment Organism
ADH
-
Yokenella sp. WZY002

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
for crotonyl alcohol oxidation Yokenella sp. WZY002
65
-
for crotonaldehyde reduction Yokenella sp. WZY002

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C Yokenella sp. WZY002

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
for crotonaldehyde reduction Yokenella sp. WZY002
8
-
for crotonyl alcohol oxidation Yokenella sp. WZY002

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Yokenella sp. WZY002
NADPH
-
Yokenella sp. WZY002