Literature summary for 1.1.1.91 extracted from

Ying, X.; Wang, Y.; Xiong, B.; Wu, T.; Xie, L.; Yu, M.; Wang, Z.
Characterization of an allylic/benzyl alcohol dehydrogenase from Yokenella sp. strain WZY002, an organism potentially useful for the synthesis of alpha,beta-unsaturated alcohols from allylic aldehydes and ketones (2014), Appl. Environ. Microbiol., 80, 2399-2409.

Search for more literature for 1.1.1.91

Activating Compound

Activating Compound	Comment	Organism	Structure
acetone	125% activity at 20% (v/v) acetone	Yokenella sp. WZY002
dithiothreitol	123% activity at 1 mM	Yokenella sp. WZY002
EDTA	110% activity at 1 mM	Yokenella sp. WZY002

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Yokenella sp. WZY002

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	complete inhibition at 1 mM	Yokenella sp. WZY002
Al3+	13.5% inhibition at 1 mM	Yokenella sp. WZY002
Co2+	80.5% inhibition at 1 mM	Yokenella sp. WZY002
Cu2+	complete inhibition at 1 mM	Yokenella sp. WZY002
Fe2+	39.7% inhibition at 1 mM	Yokenella sp. WZY002
iodoacetate	94.5% residual activity at 10 mM	Yokenella sp. WZY002
Zn2+	99.5% inhibition at 1 mM	Yokenella sp. WZY002

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.4	-	benzaldehyde	at pH 6.5 and 65°C	Yokenella sp. WZY002
0.6	-	NADP+	with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C	Yokenella sp. WZY002
0.7	-	NADP+	with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C	Yokenella sp. WZY002
3.3	-	crotonaldehyde	at pH 8.0 and 55°C	Yokenella sp. WZY002
9.1	-	crotonyl alcohol	at pH 8.0 and 55°C	Yokenella sp. WZY002
14	-	benzyl alcohol	at pH 6.5 and 65°C	Yokenella sp. WZY002

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	113% activity at 1 mM	Yokenella sp. WZY002
additional information	Ca2+, Na+, Mg2+, Mn2+, and K+ have no significant effect on the enzyme activity	Yokenella sp. WZY002
Zn2+	dependent on	Yokenella sp. WZY002

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36411	-	-	Yokenella sp. WZY002
39000	-	2 * 39000, SDS-PAGE	Yokenella sp. WZY002
75000	-	gel filtration	Yokenella sp. WZY002

Organic Solvent Stability

Organic Solvent	Comment	Organism
DMSO	the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide and also remains active in 40% (v/v)	Yokenella sp. WZY002
Ethanol	the enzyme retains more than 49% of the initial activity in 20% (v/v) ethanol and also remains active in 40% (v/v)	Yokenella sp. WZY002
isopropanol	the enzyme retains more than 49% of the initial activity in 20% (v/v) isopropanol and also remains active in 40% (v/v)	Yokenella sp. WZY002
Methanol	the enzyme retains more than 49% of the initial activity in 20% (v/v) methanol and also remains active in 40% (v/v)	Yokenella sp. WZY002

Organism

Organism	UniProt	Comment	Textmining
Yokenella sp. WZY002	W6CX26	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration	Yokenella sp. WZY002

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(E)-oct-2-enal + NADPH + H+	-	Yokenella sp. WZY002	trans-2-octen-1-ol + NADP+	-	?
1-butanol + NADP+	56% activity compared to crotyl alcohol	Yokenella sp. WZY002	butyraldehyde + NADPH + H+	-	?
1-propanol + NADP+	17.4% activity compared to crotyl alcohol	Yokenella sp. WZY002	propionaldehyde + NADPH + H+	-	?
2-hydroxyacetophenone + NADPH + H+	-	Yokenella sp. WZY002	(S)-1,2-phenylethanediol + NADP+	-	?
acetophenone + NADPH + H+	-	Yokenella sp. WZY002	?	-	r
alpha-ionone + NADPH + H+	-	Yokenella sp. WZY002	?	-	r
benzaldehyde + NADPH + H+	highest reductive activity with benzaldehyde	Yokenella sp. WZY002	benzyl alcohol + NADP+	-	r
benzyl alcohol + NADP+	41.7% activity compared to crotyl alcohol	Yokenella sp. WZY002	benzaldehyde + NADPH + H+	-	r
crotonaldehyde + NADPH + H+	-	Yokenella sp. WZY002	crotonyl alcohol + NADP+	-	r
crotonyl alcohol + NADP+	highest oxidative activity with crotyl alcohol	Yokenella sp. WZY002	crotonaldehyde + NADPH + H+	-	r
additional information	no activity with NADH or NAD+	Yokenella sp. WZY002	?	-	?
trans-4-phenyl-3-buten-2-one + NADPH + H+	-	Yokenella sp. WZY002	?	-	r
vanillin + NADPH + H+	-	Yokenella sp. WZY002	vanillic alcohol + NADP+	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 39000, SDS-PAGE	Yokenella sp. WZY002
homodimer	2 * 36411, calculated from amino acid sequence	Yokenella sp. WZY002

Synonyms

Synonyms	Comment	Organism
ADH	-	Yokenella sp. WZY002

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	for crotonyl alcohol oxidation	Yokenella sp. WZY002
65	-	for crotonaldehyde reduction	Yokenella sp. WZY002

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	65	the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C and 1.5 h at 60°C. The activity loss at 65°C is relatively rapid, and the enzyme retains 30.8% of the initial activity after 30 min of heat treatment	Yokenella sp. WZY002

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
93.3	-	benzyl alcohol	at pH 6.5 and 65°C	Yokenella sp. WZY002
101	-	crotonyl alcohol	at pH 8.0 and 55°C	Yokenella sp. WZY002
194	-	NADP+	with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C	Yokenella sp. WZY002
360	-	benzaldehyde	at pH 6.5 and 65°C	Yokenella sp. WZY002
405	-	crotonaldehyde	at pH 8.0 and 55°C	Yokenella sp. WZY002
433	-	NADP+	with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C	Yokenella sp. WZY002

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	for crotonaldehyde reduction	Yokenella sp. WZY002
8	-	for crotonyl alcohol oxidation	Yokenella sp. WZY002

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Yokenella sp. WZY002
NADPH	-	Yokenella sp. WZY002

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.6	-	benzyl alcohol	at pH 6.5 and 65°C	Yokenella sp. WZY002
11	-	crotonyl alcohol	at pH 8.0 and 55°C	Yokenella sp. WZY002
122	-	crotonaldehyde	at pH 8.0 and 55°C	Yokenella sp. WZY002
278	-	NADP+	with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C	Yokenella sp. WZY002
720	-	NADP+	with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C	Yokenella sp. WZY002
899	-	benzaldehyde	at pH 6.5 and 65°C	Yokenella sp. WZY002

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)