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Literature summary for 1.1.1.91 extracted from
- Biochemical characterization of a functional recombinant aryl-alcohol dehydrogenase from Taiwanofungus camphorata (2014), Bot. Stud., 55, 14.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | Taiwanofungus camphoratus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
veratraldehyde | at pH 6.0 temperature not specified in the publication | Taiwanofungus camphoratus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
1 * 39000, SDS-PAGE | Taiwanofungus camphoratus |
| 40580 | - |
- |
Taiwanofungus camphoratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Taiwanofungus camphoratus | V9HZX4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Taiwanofungus camphoratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Taiwanofungus camphoratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dimethoxybenzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | 2,4-dimethoxybenzyl aldehyde + NADPH + H+ | - |
? | |
| 3,4-dimethoxybenzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | ? | - |
? | |
| 3,4-dimethoxybenzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | veratraldehyde + NADPH + H+ | - |
r | |
| 4-(hydroxymethyl)benzoic acid + NAD+ | - |
Taiwanofungus camphoratus | ? + NADH + H+ | - |
? | |
| benzaldehyde + NADPH + H+ | reduction of benzaldehyde at pH 6.0 is NADPH-dependent while NADH does not yield detectable activity under the same conditions | Taiwanofungus camphoratus | benzyl alcohol + NADP+ | - |
r | |
| benzyl alcohol + NAD+ | oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent while NADP+ did not yield detectable activity under the same conditions | Taiwanofungus camphoratus | benzaldehyde + NADPH + H+ | - |
r | |
| benzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | benzaldehyde + NADPH + H+ | - |
? | |
| veratraldehyde + NADPH + H+ | - |
Taiwanofungus camphoratus | 3,4-dimethoxybenzyl alcohol + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000, SDS-PAGE | Taiwanofungus camphoratus |
| monomer | 1 * 40580, MALDI-TOF mass spectrometry | Taiwanofungus camphoratus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAD | - |
Taiwanofungus camphoratus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
the enzyme's half-life of deactivation at 58°C is 4.2 min. There is no detectable enzyme activity when it is heated above 60°C | Taiwanofungus camphoratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
with veratraldehyde as substrate | Taiwanofungus camphoratus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | at pH 8.0 and 10.0 the enzyme retains 62% activity, while the enzyme retains 13% activity at pH 4.0 | Taiwanofungus camphoratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent | Taiwanofungus camphoratus | |
| NADP+ | - |
Taiwanofungus camphoratus | |
| NADPH | reduction of benzaldehyde at pH 6.0 is NADPH-dependent | Taiwanofungus camphoratus | |
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