Cloned (Comment) | Organism |
---|---|
diverse mutants expressed in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | diverse mutants with different interaction between the 3 binding domains of each of 4 subunits and modified kinetics | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-serine | sigmoidal binding curve with mutant G294V/G336V, mutants with decreased sensitivity to serine | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
diverse mutants expressed in Escherichia coli | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-phospho-D-glycerate + NAD+ = 3-phosphooxypyruvate + NADH + H+ | model for catalytic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | subunit structure with substrate binding domain, coenzyme binding domain and regulatory domain, active sites | Escherichia coli |