Literature summary for 1.1.1.95 extracted from

Thompson, J.R.; Bell, J.K.; Bratt, J.; Grant, G.A.; Banaszak, L.J.
Vmax regulation through domain and subunit changes. The active form of phosphoglycerate dehydrogenase (2005), Biochemistry, 44, 5763-5773.

Search for more literature for 1.1.1.95

Cloned(Commentary)

Cloned (Comment)	Organism
expression as selenomethionine-labeled enzyme in strain B834	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant selenomethionine-labeled enzyme, in presence of 2 mM NAD+ and 5 mM 2-ketoglutarate, euqilibration against precipitation solution containing 1.18-1.25 M ammonium sulfate and 100 mM potassium phosphate, pH 6.4, 18°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.24 A resolution	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-phospho-D-glycerate + NAD+	Escherichia coli	first step in biosynthesis of L-serine, Vmax regulation through domain and subunit changes, overview	3-phosphohydroxypyruvate + NADH	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A9T0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant selenomethionine-labeled enzyme from strain B834	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NADH + H+	-	Escherichia coli	2-hydroxyglutarate + NAD+	-	?
3-phospho-D-glycerate + NAD+	-	Escherichia coli	3-phosphohydroxypyruvate + NADH	-	r
3-phospho-D-glycerate + NAD+	first step in biosynthesis of L-serine, Vmax regulation through domain and subunit changes, overview	Escherichia coli	3-phosphohydroxypyruvate + NADH	-	?

Subunits

Subunits	Comment	Organism
More	quarternary structure of the active enzyme, conformational changes through domain-domain reorientation, stereochemical model, overview	Escherichia coli

Synonyms

Synonyms	Comment	Organism
PGDH	-	Escherichia coli
phosphoglycerate dehydrogenase	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Escherichia coli
NADH	-	Escherichia coli

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Release 2023.1 (January 2023)