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Literature summary for 1.1.1.95 extracted from
- Metabolic engineering for betaine accumulation in microbes and plants (2007), J. Biol. Chem., 282, 34185-34193.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | - |
Aphanothece halophytica |  |
| KCl | - |
Aphanothece halophytica | |
| MgCl2 | - |
Aphanothece halophytica | |
| NaCl | PGDH activity increase about 1.5-2.3fold upon the increase of salinity from 0.5 to 2.5 M NaCl | Aphanothece halophytica | |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | results demonstrate that PGDH enhances the levels of betaine by providing the precursor serine for both choline oxidation and glycine methylation pathways | Aphanothece halophytica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli and Arabidopsis thaliana | Aphanothece halophytica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of the Aphanothece PGDH gene in Escherichia coli causes an increase in levels of betaine as well as glycine and serine. Expression of the Aphanothece PGDH gene in Arabidopsis thaliana, in which the betaine synthetic pathway is introduced via glycine methylation, further increases betaine levels and improves the stress tolerance | Aphanothece halophytica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
3-phosphohydroxypyruvate | - |
Aphanothece halophytica | |
| 0.9 | - |
3-phospho-D-glycerate | - |
Aphanothece halophytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56410 | - |
deduced from cDNA | Aphanothece halophytica |
| 57000 | - |
SDS-PAGE | Aphanothece halophytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aphanothece halophytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulphate fractionation and ion exchange chromatography | Aphanothece halophytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NAD+ | - |
Aphanothece halophytica | 3-phosphohydroxypyruvate + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | gel filtration | Aphanothece halophytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-phosphoglycerate dehydrogenase | - |
Aphanothece halophytica |
| ApPGDH | - |
Aphanothece halophytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aphanothece halophytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity remains stable after incubation at 40°C for 7 min, but decreases to 50% when the enzyme is incubated at 50°C for 7 min | Aphanothece halophytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
3-phosphohydroxypyruvate reduction | Aphanothece halophytica |
| 9 | - |
3-phospho-D-glycerate oxidation | Aphanothece halophytica |
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Release 2023.1 (January 2023)
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