Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-serine | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.189 | - |
NAD+ | isoform PGDH2, at pH 8.1 and 37°C | Arabidopsis thaliana | |
0.239 | - |
NAD+ | isoform PGDH3, at pH 8.1 and 37°C | Arabidopsis thaliana | |
0.271 | - |
NAD+ | isoform PGDH2, at pH 7.2 and 37°C | Arabidopsis thaliana | |
0.377 | - |
NAD+ | isoform PGDH1, at pH 7.2 and 37°C | Arabidopsis thaliana | |
0.39 | - |
NAD+ | isoform PGDH1, at pH 8.1 and 37°C | Arabidopsis thaliana | |
0.551 | - |
NAD+ | isoform PGDH3, at pH 7.2 and 37°C | Arabidopsis thaliana | |
0.899 | - |
3-phospho-D-glycerate | isoform PGDH2, at pH 8.1 and 37°C | Arabidopsis thaliana | |
1.006 | - |
3-phospho-D-glycerate | isoform PGDH3, at pH 8.1 and 37°C | Arabidopsis thaliana | |
1.308 | - |
3-phospho-D-glycerate | isoform PGDH1, at pH 8.1 and 37°C | Arabidopsis thaliana | |
1.926 | - |
3-phospho-D-glycerate | isoform PGDH2, at pH 7.2 and 37°C | Arabidopsis thaliana | |
2.11 | - |
3-phospho-D-glycerate | isoform PGDH1, at pH 7.2 and 37°C | Arabidopsis thaliana | |
2.559 | - |
3-phospho-D-glycerate | isoform PGDH3, at pH 7.2 and 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Arabidopsis thaliana | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O04130 | ecotype Col-0 | - |
Arabidopsis thaliana | O49485 | ecotype Col-0 | - |
Arabidopsis thaliana | Q9LT69 | ecotype Col-0 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Arabidopsis thaliana | - |
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Arabidopsis thaliana | 3-phosphonooxypyruvate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGDH1 | isoform | Arabidopsis thaliana |
PGDH2 | isoform | Arabidopsis thaliana |
PGDH3 | isoform | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.741 | - |
NAD+ | isoform PGDH1, at pH 8.1 and 37°C | Arabidopsis thaliana | |
1.741 | - |
3-phospho-D-glycerate | isoform PGDH1, at pH 8.1 and 37°C | Arabidopsis thaliana | |
97 | - |
NAD+ | isoform PGDH3, at pH 7.2 and 37°C | Arabidopsis thaliana | |
97 | - |
3-phospho-D-glycerate | isoform PGDH3, at pH 7.2 and 37°C | Arabidopsis thaliana | |
115.1 | - |
NAD+ | isoform PGDH1, at pH 7.2 and 37°C | Arabidopsis thaliana | |
115.1 | - |
3-phospho-D-glycerate | isoform PGDH1, at pH 7.2 and 37°C | Arabidopsis thaliana | |
119.7 | - |
NAD+ | isoform PGDH2, at pH 7.2 and 37°C | Arabidopsis thaliana | |
119.7 | - |
3-phospho-D-glycerate | isoform PGDH2, at pH 7.2 and 37°C | Arabidopsis thaliana | |
142.4 | - |
NAD+ | isoform PGDH3, at pH 8.1 and 37°C | Arabidopsis thaliana | |
142.4 | - |
3-phospho-D-glycerate | isoform PGDH3, at pH 8.1 and 37°C | Arabidopsis thaliana | |
147.4 | - |
NAD+ | isoform PGDH2, at pH 8.1 and 37°C | Arabidopsis thaliana | |
147.4 | - |
3-phospho-D-glycerate | isoform PGDH2, at pH 8.1 and 37°C | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | PGDH1-silenced lines are inhibited in growth | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.91 | - |
3-phospho-D-glycerate | isoform PGDH3, at pH 7.2 and 37°C | Arabidopsis thaliana | |
54.57 | - |
3-phospho-D-glycerate | isoform PGDH2, at pH 8.1 and 37°C | Arabidopsis thaliana | |
62.16 | - |
3-phospho-D-glycerate | isoform PGDH1, at pH 7.2 and 37°C | Arabidopsis thaliana | |
133.1 | - |
3-phospho-D-glycerate | isoform PGDH1, at pH 8.1 and 37°C | Arabidopsis thaliana | |
141.6 | - |
3-phospho-D-glycerate | isoform PGDH2, at pH 7.2 and 37°C | Arabidopsis thaliana | |
154 | - |
3-phospho-D-glycerate | isoform PGDH3, at pH 8.1 and 37°C | Arabidopsis thaliana | |
176.1 | - |
NAD+ | isoform PGDH3, at pH 7.2 and 37°C | Arabidopsis thaliana | |
305.7 | - |
NAD+ | isoform PGDH2, at pH 8.1 and 37°C | Arabidopsis thaliana | |
442.6 | - |
NAD+ | isoform PGDH1, at pH 7.2 and 37°C | Arabidopsis thaliana | |
446.3 | - |
NAD+ | isoform PGDH1, at pH 8.1 and 37°C | Arabidopsis thaliana | |
595 | - |
NAD+ | isoform PGDH2, at pH 7.2 and 37°C | Arabidopsis thaliana | |
780.1 | - |
NAD+ | isoform PGDH3, at pH 8.1 and 37°C | Arabidopsis thaliana |