Application | Comment | Organism |
---|---|---|
synthesis | production of L-xylulose from xylitol using a resting cell reaction leads to 35% L-xylulose within 24 h, starting from 5% xylitol as initial concentration | Aeribacillus pallidus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeribacillus pallidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
NAD+ | pH 10.0, 40°C | Aeribacillus pallidus | |
0.83 | - |
D-threitol | pH 10.0, 40°C | Aeribacillus pallidus | |
1.27 | - |
L-xylulose | pH 10.0, 40°C | Aeribacillus pallidus | |
2.06 | - |
xylitol | pH 10.0, 40°C | Aeribacillus pallidus | |
2.64 | - |
D-arabitol | pH 10.0, 40°C | Aeribacillus pallidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 50.8% of the activity with Mn2+ | Aeribacillus pallidus | |
Mg2+ | 1 mM, 36.9% of the activity with Mn2+ | Aeribacillus pallidus | |
Mn2+ | required | Aeribacillus pallidus | |
additional information | no significant stimulation by Na+, Cu2+, K+, Zn2+, Co2+ | Aeribacillus pallidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27333 | - |
x * 27333, calculated, x * 28000, SDS-PAGE | Aeribacillus pallidus |
28000 | - |
x * 27333, calculated, x * 28000, SDS-PAGE | Aeribacillus pallidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeribacillus pallidus | E0D583 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aeribacillus pallidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol + NADH + H+ | 15% conversion in resting cell reaction | Aeribacillus pallidus | D-ribulose + NAD+ | - |
? | |
D-iditol + NAD+ | 72% conversion in resting cell reaction | Aeribacillus pallidus | D-sorbose + NADH + H+ | - |
? | |
D-talitol + NADH + H+ | 15% conversion in resting cell reaction | Aeribacillus pallidus | D-tagatose + NAD+ | - |
? | |
D-threitol + NAD+ | 48% conversion in resting cell reaction | Aeribacillus pallidus | erythrulose + NADH + H+ | - |
? | |
D-xylulose + NADH + H+ | - |
Aeribacillus pallidus | xylitol + NAD+ | - |
? | |
additional information | no substrate: DL-rhamnitol, galactitol, allitol, DL-fucitol, L-iditol, L-threitol, DL-sorbitol, DL-mannitol, erythritol | Aeribacillus pallidus | ? | - |
? | |
ribitol + NADH + H+ | 15% conversion in resting cell reaction | Aeribacillus pallidus | D-ribulose + NAD+ | - |
? | |
xylitol + NAD+ | 50% conversion in resting cell reaction | Aeribacillus pallidus | L-xylulose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 27333, calculated, x * 28000, SDS-PAGE | Aeribacillus pallidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aeribacillus pallidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, 66% residual acitivity | Aeribacillus pallidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.695 | - |
D-arabitol | pH 10.0, 40°C | Aeribacillus pallidus | |
1.11 | - |
L-xylulose | pH 10.0, 40°C | Aeribacillus pallidus | |
2.75 | - |
xylitol | pH 10.0, 40°C | Aeribacillus pallidus | |
2.75 | - |
D-threitol | pH 10.0, 40°C | Aeribacillus pallidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Aeribacillus pallidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Aeribacillus pallidus | |
NAD+ | specific for NAD+ | Aeribacillus pallidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
D-arabitol | pH 10.0, 40°C | Aeribacillus pallidus | |
0.87 | - |
L-xylulose | pH 10.0, 40°C | Aeribacillus pallidus | |
1.33 | - |
xylitol | pH 10.0, 40°C | Aeribacillus pallidus | |
3.31 | - |
D-threitol | pH 10.0, 40°C | Aeribacillus pallidus |