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Literature summary for 1.1.1.B20 extracted from

  • Otagiri, M.; Kurisu, G.; Ui, S.; Takusagawa, Y.; Ohkuma, M.; Kudo, T.; Kusunoki, M.
    Crystal structure of meso-2,3-butanediol dehydrogenase in a complex with NAD+ and inhibitor mercaptoethanol at 1.7 A resolution for understanding of chiral substrate recognition mechanisms (2001), J. Biochem., 129, 205-208.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with substrate meso-2,3-butanediol and inhibitor 2-mercaptoethanol, to 1.7 A resolution. The overall structure is similar to that of other short chain dehydrogenase/reductase enzymes, the NAD+ binding site, and the positions of catalytic residues Ser139, Tyr152, and Lys156 are also conserved. 2-Mercaptoethanol forms hydrogens bonds with residues Gln 140 and Gly 183 close to the active site, which are important but not sufficient for distiguishing stereoisomerism of a chiral substrate Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
Q140I mutation mimicking the corresponding residue in (S,S)-butanediol dehydrogenase. No activity with substrates meso-butanediol or (S,S)-butanediol Klebsiella pneumoniae
Q140I/N146F mutation mimicking the corresponding residues in (S,S)-butanediol dehydrogenase. Poor activity with substrates meso-butanediol or (S,S)-butanediol Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol competitive Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(2R,3S)-butane-2,3-diol + NAD+ = acetoin + NADH + H+ catalytic triad is formed by residues Ser139, Tyr 152, and Lys156 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
meso-2,3-butanediol + NAD+
-
Klebsiella pneumoniae acetoin + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
meso-2,3-butanediol dehydrogenase
-
Klebsiella pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.52
-
2-mercaptoethanol pH 8.0, temperature not specified in the publication Klebsiella pneumoniae