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Literature summary for 1.1.1.B3 extracted from

  • Soni, P.; Kansal, H.; Banerjee, U.C.
    Purification and characterization of an enantioselective carbonyl reductase from Candida viswanathii MTCC 5158 (2007), Process Biochem., 42, 1632-1640.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2 mM, 115% of initial activity Candida viswanathii

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxy mercuribenzoate 2 mM, 5% residual activity Candida viswanathii
AgNO3 2 mM, complete inhibition Candida viswanathii
citrate 2 mM, 28% residual activity Candida viswanathii
Co(NO3)2 2 mM, 59% residual activity Candida viswanathii
CuSO4 2 mM, complete inhibition Candida viswanathii
D-cysteine 2 mM, 8% residual activity Candida viswanathii
diethyldicarbonate 2 mM, 30% residual activity Candida viswanathii
EDTA 2 mM, 49% residual activity Candida viswanathii
FeCl3 2 mM, 2% residual activity Candida viswanathii
glutathione 2 mM, 31% residual activity Candida viswanathii
guanidinium hydrochloride 1 M, 1.5% residual activity Candida viswanathii
hydroxylamine 2 mM, 23% residual activity Candida viswanathii
Hydroxyquinoline 2 mM, 18% residual activity Candida viswanathii
L-cysteine 2 mM, 12% residual activity Candida viswanathii
MgCl2 2 mM, 75% residual activity Candida viswanathii
MnSO4 2 mM, 53% residual activity Candida viswanathii
additional information not inhibitory at 2 mM: BaCl2, CaCO3, NaCl Candida viswanathii
N-ethylmaleimide 2 mM, 5% residual activity Candida viswanathii
NiCl2 2 mM, 24% residual activity Candida viswanathii
o-phenanthroline 2 mM, 9% residual activity Candida viswanathii
oxalate 2 mM, 26% residual activity Candida viswanathii
Pb(OAc)2 2 mM, 8% residual activity Candida viswanathii
potassium iodide 1 M, 1.4% residual activity Candida viswanathii
Sodium azide 2 mM, 35% residual activity Candida viswanathii
sodium dodecylsulfate 1% w/v, 6% residual activity Candida viswanathii
tartarate 2 mM, 21% residual activity Candida viswanathii
Triton X-100 1% w/v, 67% residual activity Candida viswanathii
Tween 80 1% w/v, 26.5% residual activity Candida viswanathii
Urea 1 M, 5.8% residual activity Candida viswanathii
ZnSO4 2 mM, 2% residual activity Candida viswanathii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.153
-
acetophenone pH 7.0, 25°C Candida viswanathii
0.157
-
NADH pH 7.0, 25°C Candida viswanathii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
4 * 29000, SDS-PAGE Candida viswanathii
120000
-
gel filtration Candida viswanathii

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl formamide 15% v/v, 16% residual activity Candida viswanathii
dimethyl sulfoxide 15% v/v, 16% residual activity Candida viswanathii
dioxane 34% v/V, 16% residual activity Candida viswanathii
Ethanol 15% v/v, 48% residual activity Candida viswanathii
isopropanol 15% v/v, 2.5% residual activity Candida viswanathii
Pyridine 15% v/v, 0.4% residual activity Candida viswanathii
tetrahydrofuran 15% v/v, 11% residual activity Candida viswanathii

Organism

Organism UniProt Comment Textmining
Candida viswanathii
-
-
-
Candida viswanathii MTCC 5158
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida viswanathii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
358.6
-
pH 7.0, 25°C Candida viswanathii

Storage Stability

Storage Stability Organism
-20°C, phosphate buffer, pH 7.0, presence of 2-mercaptoethanol and phenylmethyl sulfonylfluoride, stable for at least 3 months Candida viswanathii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fluoroacetophenone + NADH + H+ 102% of the activity with acetophenone Candida viswanathii (1S)-1-(2-fluorophenyl)ethanol + NAD+
-
?
2'-fluoroacetophenone + NADH + H+ 102% of the activity with acetophenone Candida viswanathii MTCC 5158 (1S)-1-(2-fluorophenyl)ethanol + NAD+
-
?
3'-fluoroacetophenone + NADH + H+ 102% of the activity with acetophenone Candida viswanathii (1S)-1-(3-fluorophenyl)ethanol + NAD+
-
?
3'-fluoroacetophenone + NADH + H+ 102% of the activity with acetophenone Candida viswanathii MTCC 5158 (1S)-1-(3-fluorophenyl)ethanol + NAD+
-
?
4'-acetoxy acetophenone + NADH + H+ 80% of the activity with acetophenone Candida viswanathii (1S)-1-(4-acetoxyphenyl)ethanol + NAD+
-
?
4'-acetoxy acetophenone + NADH + H+ 80% of the activity with acetophenone Candida viswanathii MTCC 5158 (1S)-1-(4-acetoxyphenyl)ethanol + NAD+
-
?
4'-acetyl pyridine + NADH + H+ 98% of the activity with acetophenone Candida viswanathii (1S)-1-(pyridin-4-yl)ethanol + NAD+ 102% of the activity with acetophenone, enantiomeric excess of more than 99% for (S)-configuration of product ?
4'-acetyl pyridine + NADH + H+ 98% of the activity with acetophenone Candida viswanathii MTCC 5158 (1S)-1-(pyridin-4-yl)ethanol + NAD+ 102% of the activity with acetophenone, enantiomeric excess of more than 99% for (S)-configuration of product ?
4'-bromoacetophenone + NADH + H+ 82% of the activity with acetophenone Candida viswanathii (1S)-1-(4-bromophenyl)ethanol + NAD+
-
?
4'-bromoacetophenone + NADH + H+ 82% of the activity with acetophenone Candida viswanathii MTCC 5158 (1S)-1-(4-bromophenyl)ethanol + NAD+
-
?
4'-chloroacetophenone + NADH + H+ 83% of the activity with acetophenone Candida viswanathii (1S)-1-(4-chlorophenyl)ethanol + NAD+
-
?
4'-fluoroacetophenone + NADH + H+ 117% of the activity with acetophenone Candida viswanathii (1S)-1-(4-fluorophenyl)ethanol + NAD+
-
?
4'-iodoacetophenone + NADH + H+ 31% of the activity with acetophenone Candida viswanathii (1S)-1-(4-iodophenyl)ethanol + NAD+
-
?
acetophenone + NADH + H+
-
Candida viswanathii (1S)-1-phenylethanol + NAD+ enantiomeric excess of more than 99% for (S)-configuration of product ?

Subunits

Subunits Comment Organism
tetramer 4 * 29000, SDS-PAGE Candida viswanathii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Candida viswanathii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
54% of maximum activity Candida viswanathii
75
-
61% of maximum activity Candida viswanathii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
phosphate buffer, pH 7.0, 7 h, 80% residual activity Candida viswanathii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Candida viswanathii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7 more than 80% of maximum activity Candida viswanathii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Candida viswanathii
NADH
-
Candida viswanathii