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Literature summary for 1.1.1.B3 extracted from

  • Pennacchio, A.; Esposito, L.; Zagari, A.; Rossi, M.; Raia, C.A.
    Role of Tryptophan 95 in substrate specificity and structural stability of Sulfolobus solfataricus alcohol dehydrogenase (2009), Extremophiles, 13, 751-761.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli RB791 cells Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
W95L/N249Y the mutant exhibits higher activity but decreased affinity toward aliphatic alcohols, aldehydes as well as NAD+ and NADH compared to the wild type enzyme, optimum pH is at about pH 8.6 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
(S)-2-butanol wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus
0.07
-
(S)-2-pentanol wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus
8.8
-
(S)-2-pentanol mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus
29.8
-
(S)-2-butanol mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-butanol + NAD+
-
Saccharolobus solfataricus 2-butanone + NADH + H+
-
r
(S)-2-pentanol + NAD+
-
Saccharolobus solfataricus 2-pentanone + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
ADH
-
Saccharolobus solfataricus
NAD+-dependent (S)-stereospecific alcohol dehydrogenase
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
(S)-2-butanol wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus
2.6
-
(S)-2-pentanol wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus
4.8
-
(S)-2-butanol mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus
6.7
-
(S)-2-pentanol mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharolobus solfataricus