Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli RB791 cells | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
W95L/N249Y | the mutant exhibits higher activity but decreased affinity toward aliphatic alcohols, aldehydes as well as NAD+ and NADH compared to the wild type enzyme, optimum pH is at about pH 8.6 | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
(S)-2-butanol | wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus | |
0.07 | - |
(S)-2-pentanol | wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus | |
8.8 | - |
(S)-2-pentanol | mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus | |
29.8 | - |
(S)-2-butanol | mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-butanol + NAD+ | - |
Saccharolobus solfataricus | 2-butanone + NADH + H+ | - |
r | |
(S)-2-pentanol + NAD+ | - |
Saccharolobus solfataricus | 2-pentanone + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Saccharolobus solfataricus |
NAD+-dependent (S)-stereospecific alcohol dehydrogenase | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
(S)-2-butanol | wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus | |
2.6 | - |
(S)-2-pentanol | wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus | |
4.8 | - |
(S)-2-butanol | mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus | |
6.7 | - |
(S)-2-pentanol | mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus |