Application | Comment | Organism |
---|---|---|
synthesis | the enzyme catalyzes the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, the activity is 6.2 U/mg. Using two coexisting recombinant Escherichia coli strains, in which a strain expressing glucose dehydrogenase is used as an NADPH regenerator. An optical purity of 99% (e.e.) and a maximum yield of 1240 mM (S)-4-chloro-3-hydroxybutanoate are obtained, and highest turnover number of 53900 can be achieved without adding extra NADP+/NADPH | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | P87219 | cf. EC 1.1.1.289 | - |
Candida albicans ATCC MYA-2876 | P87219 | cf. EC 1.1.1.289 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Candida albicans | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | the enzyme catalyzes the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, 99% enantiomeric excess | ? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Candida albicans ATCC MYA-2876 | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | the enzyme catalyzes the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, 99% enantiomeric excess | ? |
Synonyms | Comment | Organism |
---|---|---|
SOU1 | - |
Candida albicans |