Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. The substrate binding pocket consists of residues P122, W123, E125, S174, N179, V180, S214, P215, G217, Y218, I223, S224, D225, F226 and V227, with all of the residues lying in the flexible loop regions | Debaryomyces hansenii |
Protein Variants | Comment | Organism |
---|---|---|
N179S | about 40fold increase in specific activity | Debaryomyces hansenii |
N179S/I214F/S215G | about 50fold increase in specific activity | Debaryomyces hansenii |
S174G | about 7fold increase in specific activity | Debaryomyces hansenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
1-chloroacetophenone | mutant N179S, pH 6.5, 30°C | Debaryomyces hansenii | |
1.31 | - |
1-chloroacetophenone | mutant S174G, pH 6.5, 30°C | Debaryomyces hansenii | |
1.45 | - |
1-chloroacetophenone | mutant N179S/I214F/S215G, pH 6.5, 30°C | Debaryomyces hansenii | |
2.25 | - |
1-chloroacetophenone | wild-type, pH 6.5, 30°C | Debaryomyces hansenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Debaryomyces hansenii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloroacetophenone + NADH + H+ | - |
Debaryomyces hansenii | (1S)-2-chloro-1-phenylethanol + NAD+ | more thasn 99% enantiomeric excess | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.95 | - |
1-chloroacetophenone | wild-type, pH 6.5, 30°C | Debaryomyces hansenii | |
21.8 | - |
1-chloroacetophenone | mutant S174G, pH 6.5, 30°C | Debaryomyces hansenii | |
119 | - |
1-chloroacetophenone | mutant N179S, pH 6.5, 30°C | Debaryomyces hansenii | |
204 | - |
1-chloroacetophenone | mutant N179S/I214F/S215G, pH 6.5, 30°C | Debaryomyces hansenii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.75 | - |
1-chloroacetophenone | wild-type, pH 6.5, 30°C | Debaryomyces hansenii | |
14.2 | - |
1-chloroacetophenone | mutant S174G, pH 6.5, 30°C | Debaryomyces hansenii | |
140 | - |
1-chloroacetophenone | mutant N179S/I214F/S215G, pH 6.5, 30°C | Debaryomyces hansenii | |
295 | - |
1-chloroacetophenone | mutant N179S, pH 6.5, 30°C | Debaryomyces hansenii |