Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 1 mM, 55% residual activity | Burkholderia sp. | |
alpha,alpha'-dipyridyl | 1 71, 43% residual activity | Burkholderia sp. | |
FeCl2 | 1 mM, 81% residual activity | Burkholderia sp. | |
o-phenanthroline | 1 mM, 43% residual activity | Burkholderia sp. | |
p-chloromercuribenzoate | 1 mM, 4% residual activity | Burkholderia sp. | |
ZnCl2 | 1 mM, 73% residual activity | Burkholderia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | pH 6.0, 30°C | Burkholderia sp. | |
0.04 | - |
2-butanone | pH 6.0, 30°C | Burkholderia sp. | |
0.045 | - |
(R)-2-butanol | pH 8.5, 30°C | Burkholderia sp. | |
0.065 | - |
2-butanol | pH 8.5, 30°C | Burkholderia sp. | |
0.065 | - |
acetone | pH 6.0, 30°C | Burkholderia sp. | |
0.11 | - |
2-propanol | pH 8.5, 30°C | Burkholderia sp. | |
0.15 | - |
NAD+ | pH 8.5, 30°C | Burkholderia sp. | |
0.35 | - |
(S)-2-butanol | pH 8.5, 30°C | Burkholderia sp. | |
0.76 | - |
3-Pentanol | pH 8.5, 30°C | Burkholderia sp. | |
0.84 | - |
2-Pentanol | pH 8.5, 30°C | Burkholderia sp. | |
4 | - |
1-propanol | pH 8.5, 30°C | Burkholderia sp. | |
5.4 | - |
(R)-1,3-butanediol | pH 8.5, 30°C | Burkholderia sp. | |
6.5 | - |
ethanol | pH 8.5, 30°C | Burkholderia sp. | |
10 | - |
1-butanol | pH 8.5, 30°C | Burkholderia sp. | |
52 | - |
(S)-1,3-butanediol | pH 8.5, 30°C | Burkholderia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
4 * 37000, SDS-PAGE | Burkholderia sp. |
150000 | - |
gel filtration | Burkholderia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
growth on cholesterol or propanol as sole carbon source | - |
Burkholderia sp. AIU 652 | - |
growth on cholesterol or propanol as sole carbon source | - |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
104 | - |
pH 8.5, 30°C, substrate 2-propanol | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1,3-butanediol + NAD+ | - |
Burkholderia sp. | ? | - |
r | |
(R)-2-butanol + NAD+ | - |
Burkholderia sp. | 2-butanone + NADH + H+ | - |
r | |
(S)-1,3-butanediol + NAD+ | - |
Burkholderia sp. | ? | - |
r | |
1,2-butanediol + NAD+ | 20% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
1,2-propanediol + NAD+ | 45% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
1,3-butanediol + NAD+ | 126% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
1-butanol + NAD+ | 11% of the activity with 2-propanol | Burkholderia sp. | butanal + NADH | - |
r | |
1-butanol + NAD+ | 11% of the activity with 2-propanol | Burkholderia sp. AIU 652 | butanal + NADH | - |
r | |
1-propanol + NAD+ | 9% of the activity with 2-propanol | Burkholderia sp. | propanal + NADH | - |
r | |
2,3-butanediol + NAD+ | 73% of the activity with 2-propanol | Burkholderia sp. | ? + NADH | - |
r | |
2,3-butanediol + NAD+ | 73% of the activity with 2-propanol | Burkholderia sp. AIU 652 | ? + NADH | - |
r | |
2-butanol + NAD+ | (R)-isomer is preferred over (S)-isomer | Burkholderia sp. | 2-butanone + NADH | - |
r | |
2-butanone + NADH | - |
Burkholderia sp. | (S)-2-hydroxybutane + NAD+ | - |
? | |
2-butanone + NADH | 83% of the activity with acetone | Burkholderia sp. | 2-butanol + NAD+ | - |
r | |
2-pentanol + NAD+ | 46% of the activity with 2-propanol | Burkholderia sp. | 2-pentanone + NADH | - |
r | |
2-pentanol + NAD+ | 46% of the activity with 2-propanol | Burkholderia sp. AIU 652 | 2-pentanone + NADH | - |
r | |
2-pentanone + NADH + H+ | 44% of the activity with acetone | Burkholderia sp. | 2-pentanol + NAD+ | - |
r | |
2-propanol + NAD+ | (R)-isomer is preferred over (S)-isomer | Burkholderia sp. | acetone + NADH | - |
r | |
2-propanol + NAD+ | (R)-isomer is preferred over (S)-isomer | Burkholderia sp. AIU 652 | acetone + NADH | - |
r | |
3-pentanol + NAD+ | 23% of the activity with 2-propanol | Burkholderia sp. | 3-pentanone + NADH | - |
r | |
3-pentanone + NADH | 16% of the activity with acetone | Burkholderia sp. | 3-pentanol + NAD+ | - |
r | |
4-hydroxy-2-butanone + NADH | 95% of the activity with acetone | Burkholderia sp. | ? + NAD+ | - |
r | |
acetone + NADH | - |
Burkholderia sp. | 2-propanol + NAD+ | - |
r | |
ethanol + NAD+ | 4% of the activity with 2-propanol | Burkholderia sp. | ethanal + NADH | - |
r | |
ethanol + NAD+ | 4% of the activity with 2-propanol | Burkholderia sp. AIU 652 | ethanal + NADH | - |
r | |
hydroxyacetone + NADH | 92% of the activity with acetone | Burkholderia sp. | ? + NAD+ | - |
r | |
methylpyruvate + NADH | 64% of the activity with acetone | Burkholderia sp. | ? + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37000, SDS-PAGE | Burkholderia sp. |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase (NADH, specific for (S)-configuration of alcohol) | - |
Burkholderia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Burkholderia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
60 min, pH 7.0, 60% residual activity | Burkholderia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reduction | Burkholderia sp. |
8.5 | - |
oxidation, phosphate buffer | Burkholderia sp. |
9 | - |
and above, oxidation, Tris buffer | Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+ | Burkholderia sp. | |
NAD+ | - |
Burkholderia sp. | |
NADH | - |
Burkholderia sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Burkholderia sp. | isoelectric focusing | - |
5.4 |