Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta 2(DE3) | Metallosphaera sedula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde. Incubation with 10 mM EDTA for 16 h or with 100 mM EDTA for 1 h at temperatures up to 65°C does not inactivate the enzyme, nor does addition of 10 mM EDTA to the assay mixture inhibit enzyme activity | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | 65°C, pH 7.9 | Metallosphaera sedula | |
0.052 | - |
succinate semialdehyde | 65°C, pH 7.9, cosubstrate: NADPH | Metallosphaera sedula | |
0.18 | - |
NADH | 65°C, pH 7.9 | Metallosphaera sedula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no stimulation by Mg2+ or Mn2+ (below 5 mM), or Fe2+ (below 1 mM) | Metallosphaera sedula | |
Zn2+ | the enzyme contains 1.9 mol of zinc per mol of enzyme monomer | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Metallosphaera sedula |
74000 | - |
gel filtration | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate semialdehyde + NADPH + H+ | Metallosphaera sedula | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | 4-hydroxybutanoate + NADP+ | - |
? | |
succinate semialdehyde + NADPH + H+ | Metallosphaera sedula DSM 5348 | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | 4-hydroxybutanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YGN0 | - |
- |
Metallosphaera sedula DSM 5348 | A4YGN0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:autotrophically-grown cell | no activity in heterotrophically-grown cell | Metallosphaera sedula | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
cell extract, NADH-dependent reaction, 65°C, pH 7.9 | Metallosphaera sedula |
1.4 | - |
cell extract, NADPH-dependent reaction, 65°C, pH 7.9 | Metallosphaera sedula |
700 | - |
purified enzyme, NADPH-dependent reaction, 65°C, pH 7.9 | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate | Metallosphaera sedula | ? | - |
? | |
additional information | malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate | Metallosphaera sedula DSM 5348 | ? | - |
? | |
succinate semialdehyde + NADH + H+ | very low activity with NADH | Metallosphaera sedula | 4-hydroxybutanoate + NAD+ | - |
? | |
succinate semialdehyde + NADH + H+ | very low activity with NADH | Metallosphaera sedula DSM 5348 | 4-hydroxybutanoate + NAD+ | - |
? | |
succinate semialdehyde + NADPH + H+ | - |
Metallosphaera sedula | 4-hydroxybutanoate + NADP+ | - |
? | |
succinate semialdehyde + NADPH + H+ | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | Metallosphaera sedula | 4-hydroxybutanoate + NADP+ | - |
? | |
succinate semialdehyde + NADPH + H+ | - |
Metallosphaera sedula DSM 5348 | 4-hydroxybutanoate + NADP+ | - |
? | |
succinate semialdehyde + NADPH + H+ | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | Metallosphaera sedula DSM 5348 | 4-hydroxybutanoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
Msed_1424 | locus name | Metallosphaera sedula |
succinate semialdehyde reductase | - |
Metallosphaera sedula |
succinic semialdehyde reductase | - |
Metallosphaera sedula |
succinic semialdehyde reductase (NADPH) | - |
Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
440 | - |
NADPH | 65°C, pH 7.9 | Metallosphaera sedula | |
440 | - |
succinate semialdehyde | 65°C, pH 7.9 | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
at 20°C | Metallosphaera sedula |
7.5 | - |
at 65°C | Metallosphaera sedula |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | at 65°C, half-maximal activity at pH 6.5 and at pH 8.0 | Metallosphaera sedula |
6.9 | 8.4 | at 20°C, half-maximal activity at pH 6.9 and at pH 8.4 | Metallosphaera sedula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Metallosphaera sedula |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea | Metallosphaera sedula |