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Literature summary for 1.1.2.5 extracted from

  • Ogata, M.; Arihara, K.; Yagi, T.
    D-Lactate dehydrogenase of Desulfovibrio vulgaris (1981), J. Biochem., 89, 1423-1431.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight stimulation Desulfovibrio vulgaris

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Desulfovibrio vulgaris
cyanide
-
Desulfovibrio vulgaris
PCMB
-
Desulfovibrio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
D-lactate
-
Desulfovibrio vulgaris
2.4
-
DL-2-hydroxybutyrate
-
Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-lactate + ferricytochrome c-553 Desulfovibrio vulgaris starting enzyme for carbon and energy metabolism pyruvate + ferrocytochrome c-553
-
?

Organism

Organism UniProt Comment Textmining
Desulfovibrio vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Desulfovibrio vulgaris

Storage Stability

Storage Stability Organism
-20°C, 2 days, 20% loss of activity Desulfovibrio vulgaris
4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-lactate + 1-methoxyphenazinium methyl sulfate
-
Desulfovibrio vulgaris pyruvate + ?
-
?
D-lactate + 2,6-dichlorophenolindophenol
-
Desulfovibrio vulgaris pyruvate + ?
-
?
D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride
-
Desulfovibrio vulgaris pyruvate + ?
-
?
D-lactate + ferricyanide
-
Desulfovibrio vulgaris pyruvate + ferrocyanide
-
?
D-lactate + ferricytochrome c-553
-
Desulfovibrio vulgaris pyruvate + ferrocytochrome c-553
-
?
D-lactate + ferricytochrome c-553 starting enzyme for carbon and energy metabolism Desulfovibrio vulgaris pyruvate + ferrocytochrome c-553
-
?
D-lactate + methylene blue
-
Desulfovibrio vulgaris pyruvate + ?
-
?
D-lactate + nitrotetrazolium blue
-
Desulfovibrio vulgaris pyruvate + ?
-
?
DL-2-hydroxybutyrate + ferricytochrome c-553
-
Desulfovibrio vulgaris 2-ketobutyrate + ferrocytochrome c-553
-
?
additional information no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity Desulfovibrio vulgaris ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
D-lactate + nitrotetrazolium blue Desulfovibrio vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue Desulfovibrio vulgaris

Cofactor

Cofactor Comment Organism Structure
Ferricytochrome c-553
-
Desulfovibrio vulgaris