Cloned (Comment) | Organism |
---|---|
gene adhA, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs | Acetobacter pasteurianus |
gene adhB, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs | Acetobacter pasteurianus |
gene adhS, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs | Acetobacter pasteurianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + 2 ferricytochrome c | Acetobacter pasteurianus | - |
ethanal + 2 ferrocytochrome c | - |
? | |
ethanol + 2 ferricytochrome c | Acetobacter pasteurianus MSU10 | - |
ethanal + 2 ferrocytochrome c | - |
? | |
ethanol + 2 ferricytochrome c | Acetobacter pasteurianus IFO3191 | - |
ethanal + 2 ferrocytochrome c | - |
? | |
ethanol + 2 ferricytochrome c | Acetobacter pasteurianus SKU1108 | - |
ethanal + 2 ferrocytochrome c | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
acetic acid | ADH from strain IFO3191 retains 36% of the activities after treating with 2% acetic acid at 30°C for 30 min, and 45% after incubation with 4% acetic acid at 25°C for 30 min, unstable in 4% acetic acid at 30°C | Acetobacter pasteurianus |
acetic acid | ADH from strain MSU10 shows the highest resistance to acetic acid. The enzyme retains over 90% of the original activity after 30 min incubation with 2% acetic acid at 30°C and also with 4% acetic acid at 25°C, unstable in 4% acetic acid at 30°C | Acetobacter pasteurianus |
acetic acid | ADH from strain SKU1108 retains 61% of the activities after treating with 2% acetic acid at 30°C for 30 min, and 79% after incubation with 4% acetic acid at 25°C for 30 min, unstable in 4% acetic acid at 30°C | Acetobacter pasteurianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter pasteurianus | C7G3B7 | gene adhA encoding subunit I; isolated from passion fruit | - |
Acetobacter pasteurianus | C7G3B8 | gene adhB encoding subunit II; isolated from passion fruit | - |
Acetobacter pasteurianus | C9K501 | gene adhA encoding subunit I | - |
Acetobacter pasteurianus | C9K502 | gene adhB encoding subunit II | - |
Acetobacter pasteurianus | C9K504 | gene adhS encoding subunit III | - |
Acetobacter pasteurianus | C9W9E3 | gene adhS encoding the subunit III | - |
Acetobacter pasteurianus | D2SZY4 | gene adhA encoding the large subunit | - |
Acetobacter pasteurianus | D2SZY5 | gene adhB encoding the cytochrome c subunit | - |
Acetobacter pasteurianus IFO3191 | C9K501 | gene adhA encoding subunit I | - |
Acetobacter pasteurianus IFO3191 | C9K502 | gene adhB encoding subunit II | - |
Acetobacter pasteurianus IFO3191 | C9K504 | gene adhS encoding subunit III | - |
Acetobacter pasteurianus MSU10 | C7G3B7 | gene adhA encoding subunit I; isolated from passion fruit | - |
Acetobacter pasteurianus MSU10 | C7G3B8 | gene adhB encoding subunit II; isolated from passion fruit | - |
Acetobacter pasteurianus SKU1108 | C9W9E3 | gene adhS encoding the subunit III | - |
Acetobacter pasteurianus SKU1108 | D2SZY4 | gene adhA encoding the large subunit | - |
Acetobacter pasteurianus SKU1108 | D2SZY5 | gene adhB encoding the cytochrome c subunit | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain IFO3191 by ultracentrifugation and anion exchange chromatography | Acetobacter pasteurianus |
native enzyme from strain MSU10 by ultracentrifugation and anion exchange chromatography | Acetobacter pasteurianus |
native enzyme from strain SKU1108 by ultracentrifugation and anion exchange chromatography | Acetobacter pasteurianus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37°C than strain SKU1108, while strain SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37°C of these thermotolerant strains is superior to that of mesophilic strain IFO3191 having weak growth and very delayed acetic acid production at 37°C even at 4% ethanol | Acetobacter pasteurianus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus | ethanal + 2 ferrocytochrome c | - |
? | |
ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus MSU10 | ethanal + 2 ferrocytochrome c | - |
? | |
ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus IFO3191 | ethanal + 2 ferrocytochrome c | - |
? | |
ethanol + 2 ferricytochrome c | - |
Acetobacter pasteurianus SKU1108 | ethanal + 2 ferrocytochrome c | - |
? |
Synonyms | Comment | Organism |
---|---|---|
quinoprotein alcohol dehydrogenase | - |
Acetobacter pasteurianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Acetobacter pasteurianus |
35 | - |
- |
Acetobacter pasteurianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Acetobacter pasteurianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Acetobacter pasteurianus |
General Information | Comment | Organism |
---|---|---|
physiological function | the ADH involved in ethanol oxidation of the thermotolerant strain is important for the high temperature fermentation | Acetobacter pasteurianus |