Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyanide | suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview | Paracoccus denitrificans | |
NH4+ | obligatory activator | Paracoccus denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview | Paracoccus denitrificans | |
N,N,N',N'-tetramethyl-4-phenylenediamine | i.e. Wurster's blue | Paracoccus denitrificans | |
phenazine ethosulfate | the articficial cofactor exhibits strong substrate inhibition | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
methanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans | |
3.22 | - |
N,N,N',N'-tetramethyl-4-phenylenediamine | pH 9.0 30°C | Paracoccus denitrificans | |
3.39 | - |
ethanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans | |
10.7 | - |
Propanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans | |
11.8 | - |
phenazine ethosulfate | pH 9.0, 30°C | Paracoccus denitrificans | |
91.4 | - |
butanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + ferricytochrome c551i | Paracoccus denitrificans | - |
formaldehyde + ferrocytochrome c551i | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | P12293 | P12293 (large subunit, alpha) and P12293 (small subunit, beta) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | butyraldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? | |
ethanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | acetaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? | |
methanol + 2,6-dichlorophenolindophenol | - |
Paracoccus denitrificans | formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
methanol + ferricytochrome c551i | - |
Paracoccus denitrificans | formaldehyde + ferrocytochrome c551i | - |
? | |
methanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | formaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? | |
methanol + phenazine ethosulfate | phenazine ethosulfate is a two-electron acceptor | Paracoccus denitrificans | formaldehyde + reduced phenazine ethosulfate | - |
? | |
propanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | propionaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
quinoprotein methanol dehydrogenase | - |
Paracoccus denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Paracoccus denitrificans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
phenazine ethosulfate | pH 9.0, 30°C | Paracoccus denitrificans | |
9.37 | - |
N,N,N',N'-tetramethyl-4-phenylenediamine | pH 9.0 30°C | Paracoccus denitrificans |