Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.10 extracted from

  • Schaefer, A.; Bieg, S.; Huwig, A.; Kohring, G.W.; Giffhorn, F.
    Purification by immunoaffinity chromatography, characterization, and structural analysis of a thermostable pyranose oxidase from the white rot fungus Phlebiopsis gigantea (1996), Appl. Environ. Microbiol., 62, 2586-2592.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.10

Inhibitors

Inhibitors Comment Organism Structure
N-bromosuccinimide completely inhibitory at 1 mM Phlebiopsis gigantea
p-chloromercuribenzoate completely inhibits at 1 mM Phlebiopsis gigantea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 D-glucose
-
 Phlebiopsis gigantea
16.5
-
 L-sorbose
-
 Phlebiopsis gigantea
22.2
-
 D-xylose
-
 Phlebiopsis gigantea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy Phlebiopsis gigantea
77000
-
 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy Phlebiopsis gigantea
273700
-
 calculation on the basis of the Stoke's radius and sedimentation coefficient Phlebiopsis gigantea
280000
-
 gel filtration Phlebiopsis gigantea
305000
-
 native gradient gel electrophoresis Phlebiopsis gigantea
323600
-
 electron microscopy Phlebiopsis gigantea

Organism

Organism UniProt Comment Textmining
Phlebiopsis gigantea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using heat treatment, immunoaffinity chromatography and gel filtration on Superdex 200 Phlebiopsis gigantea

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.15
-
 purified enzyme Phlebiopsis gigantea

Storage Stability

Storage Stability Organism
40°C, 10 mM sodium phosphate, pH 6-8, half life of enzyme activity: more than 300 days Phlebiopsis gigantea
50°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: more than 110 days Phlebiopsis gigantea
65°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: 1 h Phlebiopsis gigantea
inactivated by freezing and thawing Phlebiopsis gigantea
room temperature and 30°C, pH 5-10, half life of enzyme activity: more than 300 days Phlebiopsis gigantea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + O2 8.5% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-D-galactose + H2O2
-
 ?
D-glucose + O2 100% relative activity Phlebiopsis gigantea D-glucosone + H2O2
-
 ?
D-glucose + O2 the activation energy for D-glucose activation is 24.7 kJ/mol Phlebiopsis gigantea D-glucosone + H2O2
-
 ?
D-glucose + O2 D-glucose is the preferred substrate Phlebiopsis gigantea D-glucosone + H2O2
-
 ?
D-xylose + O2 43.7% relative activity to D-glucose Phlebiopsis gigantea D-xylosone + H2O2
-
 ?
L-sorbose + O2 96.3% relative activity to D-glucose Phlebiopsis gigantea 5-dehydro-D-fructose + H2O2
-
 ?
methyl alpha-D-glucoside + O2 3% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-alpha-D-methylglucoside + H2O2
-
 ?
methyl beta-D-glucoside + O2 26.3% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-beta-D-methylglucoside + H2O2
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy Phlebiopsis gigantea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Phlebiopsis gigantea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8 in 100 mM sodium phosphate Phlebiopsis gigantea
5 7.5 in 100 mM potassium phosphate Phlebiopsis gigantea

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Phlebiopsis gigantea
FAD 3.12 mol FAD per mol of enzyme Phlebiopsis gigantea