Cloned (Comment) | Organism |
---|---|
- |
Tricholoma matsutake |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.28 | - |
D-glucose | pH 7.0, 37°C | Tricholoma matsutake | |
10.7 | - |
1,5-anhydro-D-glucitol | pH 7.0, 37°C | Tricholoma matsutake | |
26.4 | - |
L-sorbose | pH 7.0, 37°C | Tricholoma matsutake | |
45 | - |
D-galactose | pH 7.0, 37°C | Tricholoma matsutake | |
45.8 | - |
D-xylose | pH 7.0, 37°C | Tricholoma matsutake | |
174 | - |
D-arabinose | pH 7.0, 37°C | Tricholoma matsutake | |
192 | - |
trehalose | pH 7.0, 37°C | Tricholoma matsutake | |
295 | - |
D-mannose | pH 7.0, 37°C | Tricholoma matsutake | |
329 | - |
D-maltose | pH 7.0, 37°C | Tricholoma matsutake |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
4 * 62000, SDS-PAGE | Tricholoma matsutake |
250000 | - |
gel filtration | Tricholoma matsutake |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tricholoma matsutake | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tricholoma matsutake |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Tricholoma matsutake | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.8 | - |
- |
Tricholoma matsutake |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M Tris-HCl, pH 7.0, enzyme retains 78% of the initial activity after 6 months in absence of glycerol | Tricholoma matsutake |
4°C, pH 7.0, enzyme retains more than 90% of the activity | Tricholoma matsutake |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-glucitol + O2 | 18.4% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
D-arabinose + O2 | 1.87% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
D-galactose + O2 | 5.41% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
D-glucose + O2 | - |
Tricholoma matsutake | D-glucosone + H2O2 | - |
? | |
D-maltose + O2 | 15.1% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
D-mannose + O2 | 6.02% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
D-xylose + O2 | 13.4% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
L-sorbose + O2 | 17.9% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? | |
trehalose + O2 | 14.3% of the activity with D-glucose | Tricholoma matsutake | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62000, SDS-PAGE | Tricholoma matsutake |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Tricholoma matsutake |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 65% of maximal activity, 70°C: about 35% of maximal activity | Tricholoma matsutake |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, stable | Tricholoma matsutake |
50 | - |
30 min, about 5% loss of activity | Tricholoma matsutake |
55 | - |
30 min, about 40% loss of activity | Tricholoma matsutake |
60 | - |
30 min, about 45% loss of activity | Tricholoma matsutake |
65 | - |
30 min, about 60% loss of activity | Tricholoma matsutake |
70 | - |
30 min, about 65% loss of activity | Tricholoma matsutake |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.79 | - |
D-arabinose | pH 7.0, 37°C | Tricholoma matsutake | |
22.5 | - |
D-galactose | pH 7.0, 37°C | Tricholoma matsutake | |
25.1 | - |
D-mannose | pH 7.0, 37°C | Tricholoma matsutake | |
55.8 | - |
D-xylose | pH 7.0, 37°C | Tricholoma matsutake | |
59.6 | - |
trehalose | pH 7.0, 37°C | Tricholoma matsutake | |
62.9 | - |
D-maltose | pH 7.0, 37°C | Tricholoma matsutake | |
74.6 | - |
L-sorbose | pH 7.0, 37°C | Tricholoma matsutake | |
76.7 | - |
1,5-anhydro-D-glucitol | pH 7.0, 37°C | Tricholoma matsutake | |
111 | - |
D-glucose | pH 7.0, 37°C | Tricholoma matsutake |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Tricholoma matsutake |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11 | pH 5.5: about 40% of maximal activity, pH 11: about 40% of maximal activity | Tricholoma matsutake |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
37°C, 30 min, 60% loss of activity | Tricholoma matsutake |
5.5 | - |
37°C, 30 min, about 15% loss of activity | Tricholoma matsutake |
7 | 11 | 37°C, 30 min, stable | Tricholoma matsutake |