Application | Comment | Organism |
---|---|---|
synthesis | development of a simple and efficient synthesis for 4-pyridoxolactone starting with pyridoxine and using a whole-cell biotransformation by two transformed Escherichia coli cell type expressing the pyridoxine oxidase and the catalase, and chaperonin, while the second set expressed pyridoxal 4-dehydrogenase, overview. Pyridoxine is first oxidized to pyridoxal, which is then dehydrogenated to 4-pyridoxolactone by pyridoxine 4-oxidase and pyridoxal 4-dehydrogenase, respectively | Microbacterium luteolum |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strains JM109 and BL21(DE3), co-expression with catalase, chaperonin, and pyridoxal 4-dehydrogenase for construction of a biotransformation system catalyzing the formation of 4-pyridoxolactone from pyridoxine in whole cells, overview | Microbacterium luteolum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxine + O2 | Microbacterium luteolum | - |
pyridoxal + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium luteolum | Q9AJD6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
recombinant enzyme in Escherichia coli cells | Microbacterium luteolum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxine + O2 | - |
Microbacterium luteolum | pyridoxal + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNOX | - |
Microbacterium luteolum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Microbacterium luteolum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Microbacterium luteolum |