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Literature summary for 1.1.3.15 extracted from

  • Yorita, K.; Aki, K.; Ohkuma-Soyejima, T.; Kokubo, T.; Misaki, H.; Massey, V.
    Conversion of L-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine (1996), J. Biol. Chem., 271, 28300-28305.
    View publication on PubMed
Search for more literature for 1.1.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH1 Aerococcus viridans

Protein Variants

Protein Variants Comment Organism
A95G much enhanced oxidase activity with longer chain L-alpha-hydroxyacids such as alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid and L-mandelic acid Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 L-Mandelate
-
 Aerococcus viridans
0.87
-
 (S)-lactate
-
 Aerococcus viridans
5
-
 DL-glycerate
-
 Aerococcus viridans
5.5
-
 DL-alpha-hydroxy-n-valerate
-
 Aerococcus viridans
6.75
-
 (S)-lactate A95G mutant Aerococcus viridans
10
-
 DL-alpha-hydroxy-n-valerate A95G mutant Aerococcus viridans
18
-
 DL-alpha-hydroxy-n-butyrate
-
 Aerococcus viridans
20
-
 L-Mandelate A95G mutant Aerococcus viridans
27
-
 DL-alpha-hydroxy-n-butyrate A95G mutant Aerococcus viridans
53
-
 DL-glycerate A95G mutant Aerococcus viridans
125
-
 L-alpha-hydroxy-isovalerate A95G mutant Aerococcus viridans
140
-
 L-alpha-hydroxy-beta-methylvalerate A95G mutant Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-3-methylvalerate + O2 A95G-mutant Aerococcus viridans ?
-
 ?
2-hydroxybutyrate + O2 wild-type and A95G-mutant Aerococcus viridans ?
-
 ?
2-hydroxyisovalerate + O2 A95G-mutant Aerococcus viridans ?
-
 ?
2-hydroxyvalerate + O2 wild-type and A95G-mutant Aerococcus viridans ?
-
 ?
DL-glycerate + O2
-
 Aerococcus viridans ?
-
 r
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol
-
 Aerococcus viridans 3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
L-alpha-hydroxy-isovalerate + O2
-
 Aerococcus viridans 3-methyl-2-oxobutanoate + H2O2
-
 r
L-mandelate + O2 A95G-mutant is also reactive Aerococcus viridans ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Aerococcus viridans