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Literature summary for 1.1.3.15 extracted from
- Distribution and properties of glycolate oxidase from bundle sheath and mesophyll cells of green amaranth leaves (Amaranthus retroflecsus) (2005), Russ. J. Plant Physiol., 52, 553-558.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| isocitrate | - |
Amaranthus retroflexus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| succinate | - |
Amaranthus retroflexus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
glycolate | pH 7.5, mesophyll cell isozyme | Amaranthus retroflexus | |
| 0.058 | - |
glycolate | pH 7.5, bundle sheath cell isozyme | Amaranthus retroflexus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Amaranthus retroflexus | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE | Amaranthus retroflexus |
| 44000 | - |
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE | Amaranthus retroflexus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycolate + O2 | Amaranthus retroflexus | - |
glyoxylate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amaranthus retroflexus | - |
green amaranth | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| tissue-specific isozymes from leaves 63.5fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, bundle sheath cell isozyme and mesophyll cell isozyme to homogeneity | Amaranthus retroflexus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bundle sheath | main location of the enzyme activity | Amaranthus retroflexus | - |
| leaf | from 18-days-old seedlings | Amaranthus retroflexus | - |
| mesophyll | - |
Amaranthus retroflexus | - |
| additional information | one isoform is located in bundle sheath cells, the second isozyme has dual location, and the third one is located in mesophyll cells | Amaranthus retroflexus | - |
| seedling | - |
Amaranthus retroflexus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.55 | - |
purified mesophyll cell isozyme | Amaranthus retroflexus |
| 0.62 | - |
purified whole leaf enzyme | Amaranthus retroflexus |
| 1.33 | - |
purified bundle sheath cell isozyme | Amaranthus retroflexus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycolate + O2 | - |
Amaranthus retroflexus | glyoxylate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE | Amaranthus retroflexus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycolate oxidase | - |
Amaranthus retroflexus |
| GO | - |
Amaranthus retroflexus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Amaranthus retroflexus | |
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