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Literature summary for 1.1.3.15 extracted from

  • Murray, M.S.; Holmes, R.P.; Lowther, W.T.
    Active site and loop 4 movements within human glycolate oxidase implications for substrate specificity and drug design (2008), Biochemistry, 47, 2439-2449.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of recombinant GO complexed with sulfate, glyoxylate, and 4-carboxy-5-dodecylsulfanyl-1,2,3-triazole (CDST), determined by X-ray crystallography are reported. A loop region (loop 4), is completely visible when the GO active site contains a small ligand. Lack of electron density for this loop in the GOCDST complex, mimicking a large substrate, indicates that a disordered to ordered transition occurs with substrate binding. Conformational flexibility of Trp110 is responsible for enabling GO to react with R-hydroxy acids. Movement of Trp110 disrupts a hydrogen-bonding network between Trp110, Leu191, Tyr134, and Tyr208. This loss indicates that active site movements are directly linked to changes in the conformation of loop 4 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-carboxy-5-dodecylsulfanyl-1,2,3-triazole
-
Homo sapiens
glyoxylate substrate inhibition at concentrations above 4 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
2-oxo-octanoate
-
Homo sapiens
0.141
-
glycolate
-
Homo sapiens
2.2
-
glyoxylate
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UJM8
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni-NTA-column, gel filtration and ion-exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyoctanoate + 2,6-dichlorophenolindophenol
-
Homo sapiens 2-oxo-octanoate + reduced 2,6-dichlorophenolindophenol
-
r
glycolate + 2,6-dichlorophenolindophenol
-
Homo sapiens glyoxylate + reduced 2,6-dichlorophenolindophenol
-
?
glyoxylate + 2,6-dichlorophenolindophenol
-
Homo sapiens ? + reduced 2,6-dichlorophenolindophenol
-
?

Synonyms

Synonyms Comment Organism
glycolate oxidase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
2-oxo-octanoate
-
Homo sapiens
0.7
-
glyoxylate
-
Homo sapiens
4.1
-
glycolate
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FMN
-
Homo sapiens