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Literature summary for 1.1.3.17 extracted from

  • Salvi, F.; Rodriguez, I.; Hamelberg, D.; Gadda, G.
    Role of F357 as an oxygen gate in the oxidative half-reaction of choline oxidase (2016), Biochemistry, 55, 1473-1484.
    View publication on PubMed
Search for more literature for 1.1.3.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Arthrobacter globiformis

Protein Variants

Protein Variants Comment Organism
F357A the mutant shows about 240fold reduced catalytic efficiency compared to the wild type enzyme Arthrobacter globiformis
M62A the mutant shows about 3fold reduced catalytic efficiency compared to the wild type enzyme Arthrobacter globiformis
M62A/F357A the enzyme shows a lack of the isomerization detected in wild type choline oxidase, and a lack of saturation with an oxygen concentration as high as 1 mM, while most other kinetic parameters are similar to those of wild type choline oxidase Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 choline wild type enzyme, at pH 7.0 and 25°C Arthrobacter globiformis
0.8
-
 choline mutant enzyme M62A, at pH 7.0 and 25°C Arthrobacter globiformis
9
-
 choline mutant enzyme M62A/F357A, at pH 7.0 and 25°C Arthrobacter globiformis
12
-
 choline mutant enzyme F357A, at pH 7.0 and 25°C Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + O2 + H2O Arthrobacter globiformis
-
 betaine + H2O2
-
 ?
choline + O2 Arthrobacter globiformis
-
 betaine aldehyde + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis Q7X2H8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + O2 + H2O
-
 Arthrobacter globiformis betaine + H2O2
-
 ?
choline + O2
-
 Arthrobacter globiformis betaine aldehyde + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
choline:oxygen 1-reductase
-
 Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 choline mutant enzyme F357A, at pH 7.0 and 25°C Arthrobacter globiformis
6.8
-
 choline mutant enzyme M62A/F357A, at pH 7.0 and 25°C Arthrobacter globiformis
12.5
-
 choline mutant enzyme M62A, at pH 7.0 and 25°C Arthrobacter globiformis
20
-
 choline wild type enzyme, at pH 7.0 and 25°C Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.21
-
 choline mutant enzyme F357A, at pH 7.0 and 25°C Arthrobacter globiformis
0.8
-
 choline mutant enzyme M62A/F357A, at pH 7.0 and 25°C Arthrobacter globiformis
16
-
 choline mutant enzyme M62A, at pH 7.0 and 25°C Arthrobacter globiformis
50
-
 choline wild type enzyme, at pH 7.0 and 25°C Arthrobacter globiformis