Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxyphenylethyl alcohol | - |
Penicillium simplicissimum | |
cinnamyl alcohol | strong competitive inhibitor of vanillyl-alcohol oxidation | Penicillium simplicissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
O2 | - |
Penicillium simplicissimum | |
0.043 | - |
phenazine methosulfate | - |
Penicillium simplicissimum | |
0.73 | - |
Vanillyl alcohol | - |
Penicillium simplicissimum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
8 * 65000, SDS-PAGE | Penicillium simplicissimum |
520000 | - |
gel filtration | Penicillium simplicissimum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium simplicissimum | - |
- |
- |
Oxidation Stability | Organism |
---|---|
photoreduction of the enzyme in the presence of the competitive inhibitor cinnamyl alcohol gives rise to a complete irreversible bleaching of the flavin spectrum | Penicillium simplicissimum |
Purification (Comment) | Organism |
---|---|
- |
Penicillium simplicissimum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:veratryl alcohol-grown cell | sole source of carbon and energy | Penicillium simplicissimum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.13 | - |
- |
Penicillium simplicissimum |
Storage Stability | Organism |
---|---|
-20°C or 4°C, in 20% (NH4)2SO4, pH 7.0, stable for several weeks | Penicillium simplicissimum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzyl alcohol + O2 | at 75% of the activity with vanillyl alcohol | Penicillium simplicissimum | 4-hydroxybenzaldehyde + H2O2 | - |
? | |
vanillyl alcohol + O2 | - |
Penicillium simplicissimum | vanillin + H2O2 | - |
? | |
vanillyl alcohol + phenazine methosulfate | - |
Penicillium simplicissimum | vanillin + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 65000, SDS-PAGE | Penicillium simplicissimum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Penicillium simplicissimum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
90 min, stable | Penicillium simplicissimum |
60 | - |
45 min, 50% loss of activity | Penicillium simplicissimum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10.5 | - |
Penicillium simplicissimum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | about 30% of maximal activity at pH 7 and pH 12 | Penicillium simplicissimum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
8alpha-(N3-Histidyl)-FAD | the octamer contains 1 mol of covalently-bound flavin per mol of subunit | Penicillium simplicissimum |