Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method, apoenzyme, ADP-complex and holoenzyme. Crystal structure of both the holo and apo form of H61T are highly similar to the structure of the wild-type enzyme | Penicillium simplicissimum |
Protein Variants | Comment | Organism |
---|---|---|
H61T | in the mutant enzyme the covalent His-C8alpha-flavin linkage is not formed, while the enzyme is still able to bind FAD and perform catalysis. The mutant enzyme is about 10fold less active with 4-(methoxymethyl)phenol than the wild-type enzyme. Crystal structure of both the holo and apo form of H61T are highly similar to the structure of the wild-type enzyme | Penicillium simplicissimum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive | Penicillium simplicissimum | |
AMP | - |
Penicillium simplicissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme H61T | Penicillium simplicissimum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium simplicissimum | P56216 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(methoxymethyl)phenol + O2 | - |
Penicillium simplicissimum | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme H61T | Penicillium simplicissimum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound. Covalent flavinylation is an autocatalytical process in which His61 plays a crucial role by activating His422 | Penicillium simplicissimum |