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Literature summary for 1.1.3.4 extracted from
- Comparison of Penicillium amagasakiense glucose oxidase purified as glyco- and aglyco-proteins (1991), FEMS Microbiol. Lett., 78, 221-226.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | values for glyco-enzyme and aglyco-enzyme | Penicillium amagasakiense |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000, aglyco-GOD, SDS-PAGE | Penicillium amagasakiense |
| 70000 | - |
2 * 70000, glyco-GOD, SDS-PAGE | Penicillium amagasakiense |
| 130000 | 150000 | aglyco-GOD, gel filtration, native PAGE | Penicillium amagasakiense |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | Penicillium amagasakiense | - |
D-glucono-1,5-lactone + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium amagasakiense | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | no significant differences in catalytic properties of glyco- and aglyco-GOD | Penicillium amagasakiense |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isolation of glyco-GOD and aglyco-GOD from tunicamycin containing growth medium | Penicillium amagasakiense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | - |
Penicillium amagasakiense | D-glucono-1,5-lactone + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 70000, glyco-GOD, SDS-PAGE | Penicillium amagasakiense |
| dimer | 2 * 60000, aglyco-GOD, SDS-PAGE | Penicillium amagasakiense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable up to | Penicillium amagasakiense |
| 45 | - |
30 min, considerable inactivation | Penicillium amagasakiense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
glyco-GOD | Penicillium amagasakiense |
| 6 | - |
aglyco-GOD | Penicillium amagasakiense |
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Release 2023.1 (January 2023)
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