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Literature summary for 1.1.3.44 extracted from

  • Clausnitzer, D.; Piepersberg, W.; Wehmeier, U.F.
    The oxidoreductases LivQ and NeoQ are responsible for the different 6-modifications in the aminoglycosides lividomycin and neomycin (2011), J. Appl. Microbiol., 111, 642-651.
    View publication on PubMed
Search for more literature for 1.1.3.44

Application

Application Comment Organism
analysis development of a coupled enzyme assays including 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide and phenazine methosulfate, resulting in the production of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide formazan which can be monitored at 570 nm Streptomyces lividus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces fradiae
expression in Escherichia coli Streptomyces lividus

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae Q53U15
-
-
Streptomyces fradiae NCIMB 8233 Q53U15
-
-
Streptomyces lividus Q2MF66
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
 pH 7.5, temperature not specified in the publication Streptomyces lividus
6.4
-
 pH 7.5, temperature not specified in the publication Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'''-N-acetyl-6'''-hydroxyneomycin C + O2 about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C Streptomyces fradiae 2'''-N-acetyl-6'''-oxoneomycin C + H2O2
-
 ?
2'''-N-acetyl-6'''-hydroxyneomycin C + O2 less than 10% of the rate with 6'''-deamino-6'''-hydroxyneomycin C Streptomyces lividus 2'''-N-acetyl-6'''-oxoneomycin C + H2O2
-
 ?
2'''-N-acetyl-6'''-hydroxyneomycin C + O2 about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C Streptomyces fradiae NCIMB 8233 2'''-N-acetyl-6'''-oxoneomycin C + H2O2
-
 ?
6'''-deamino-6'''-hydroxyneomycin C + O2
-
 Streptomyces fradiae 6'''-deamino-6'''-oxoneomycin C + H2O2
-
 ?
6'''-deamino-6'''-hydroxyneomycin C + O2
-
 Streptomyces lividus 6'''-deamino-6'''-oxoneomycin C + H2O2
-
 ?
6'''-deamino-6'''-hydroxyneomycin C + O2
-
 Streptomyces fradiae NCIMB 8233 6'''-deamino-6'''-oxoneomycin C + H2O2
-
 ?
additional information enzyme does not catalyze the reaction of EC 1.1.3.43, paromamine 6'-oxidase Streptomyces lividus ?
-
 ?

Synonyms

Synonyms Comment Organism
LivQ
-
 Streptomyces lividus
neoQ
-
 Streptomyces fradiae