Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces coelicolor |
expressed in Escherichia coli BL21(DE3) cells | Amycolatopsis orientalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-hydroxymandelate + O2 | Streptomyces coelicolor | - |
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? | |
(S)-4-hydroxymandelate + O2 | Amycolatopsis orientalis | - |
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? | |
(S)-4-hydroxymandelate + O2 | Streptomyces coelicolor CGMCC 4.1092 | - |
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? | |
(S)-4-hydroxymandelate + O2 | Amycolatopsis orientalis ATCC 19795 | - |
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | - |
- |
- |
Amycolatopsis orientalis ATCC 19795 | - |
- |
- |
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor CGMCC 4.1092 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap affinity column chromatography | Streptomyces coelicolor |
HisTrap affinity column chromatography | Amycolatopsis orientalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
310 | - |
pH and temperature not specified in the publication | Amycolatopsis orientalis |
1822 | - |
pH and temperature not specified in the publication | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-hydroxymandelate + O2 | - |
Streptomyces coelicolor | 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? | |
(S)-4-hydroxymandelate + O2 | - |
Amycolatopsis orientalis | 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? | |
(S)-4-hydroxymandelate + O2 | - |
Streptomyces coelicolor CGMCC 4.1092 | 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? | |
(S)-4-hydroxymandelate + O2 | - |
Amycolatopsis orientalis ATCC 19795 | 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HmO | - |
Streptomyces coelicolor |
HmO | - |
Amycolatopsis orientalis |
L-4-hydroxymandelate oxidase | - |
Streptomyces coelicolor |
L-4-hydroxymandelate oxidase | - |
Amycolatopsis orientalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces coelicolor |
60 | - |
- |
Amycolatopsis orientalis |