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Literature summary for 1.1.3.5 extracted from

  • Ogasawara, K.; Yamada, K.; Hatsugai, N.; Imada, C.; Nishimura, M.
    Hexose oxidase-mediated hydrogen peroxide as a mechanism for the antibacterial activity in the red seaweed Ptilophora subcostata (2016), PLoS ONE, 11, e0149084 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + O2 Ptilophora subcostata
-
 D-galactono-1,5-lactone + H2O2
-
 ?
D-glucose + O2 Ptilophora subcostata
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Ptilophora subcostata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, followed by anion exchange chromatography and gel filtration Ptilophora subcostata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 H2O2 producing activities in different algaeal species, and with different substrates, overview Ptilophora subcostata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + O2
-
 Ptilophora subcostata ? + H2O2
-
 ?
D-galactose + O2
-
 Ptilophora subcostata D-galactono-1,5-lactone + H2O2
-
 ?
D-galactose + O2 best substrate Ptilophora subcostata D-galactono-1,5-lactone + H2O2
-
 ?
D-glucose + O2
-
 Ptilophora subcostata D-glucono-1,5-lactone + H2O2
-
 ?
lactose + O2
-
 Ptilophora subcostata ? + H2O2
-
 ?
maltose + O2
-
 Ptilophora subcostata ? + H2O2
-
 ?
additional information ferrous oxidation-xylenol orange (FOX) assay Ptilophora subcostata ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000 + x * 40000, two bands from purified enzyme in SDS-PAGE, the 29 kDa form seems to be the active/mature enzyme form Ptilophora subcostata

Synonyms

Synonyms Comment Organism
Hox
-
 Ptilophora subcostata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ptilophora subcostata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 purified native enzyme, pH 7.2, 10 min, completely stable at Ptilophora subcostata
60 70 purified native enzyme, pH 7.2, 10 min, 50% activity is retained Ptilophora subcostata
80
-
 purified native enzyme, pH 7.2, 10 min, inactivation Ptilophora subcostata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Ptilophora subcostata

General Information

General Information Comment Organism
physiological function hexose oxidase-mediated hydrogen peroxide is the mechanism for the antibacterial activity, e.g. against Bacillus subtilis strain ATCC 6633, in the red seaweed Ptilophora subcostata. Crude algaeal extract prevents the growth of gram-positive and -negative bacteria, overview, which is completely inhibited by treatment with catalase. But the extract does not affect the growth of either a yeast or a filamentous fungus, such as Candida albicans and Penicillium decambens. The activity is highest in the presence of galactose. Enzyme HOX-mediated H2O2 production represents a defense mechanism against a broad range of bacteria in marine algae Ptilophora subcostata