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Literature summary for 1.1.3.6 extracted from

  • Vrielink, A.; Ghisla, S.
    Cholesterol oxidase: biochemistry and structural features (2009), FEBS J., 276, 6826-6843.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.07
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.11
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.14
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.17
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Streptomyces hygroscopicus
0.2
-
cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.2
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.2
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
0.2
-
pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.2
-
pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.2
-
pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.2
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.25
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.25
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
0.3
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.4
-
cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.4
-
pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.5
-
trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.7
-
cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.8
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
0.8
-
trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.9
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1.2
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum

Organism

Organism UniProt Comment Textmining
Brevibacterium sterolicum
-
-
-
Streptomyces hygroscopicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholestanol + O2
-
Streptomyces hygroscopicus ?
-
?
cholestanol + O2
-
Brevibacterium sterolicum ?
-
?
cholesterol + O2
-
Streptomyces hygroscopicus cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
?
pregnenolone + O2
-
Streptomyces hygroscopicus ?
-
?
pregnenolone + O2
-
Brevibacterium sterolicum ?
-
?
trans-androsterone + O2
-
Streptomyces hygroscopicus ?
-
?
trans-androsterone + O2
-
Brevibacterium sterolicum ?
-
?
trans-dehydroandrosterone + O2
-
Streptomyces hygroscopicus ?
-
?
trans-dehydroandrosterone + O2
-
Brevibacterium sterolicum ?
-
?

Synonyms

Synonyms Comment Organism
BsChOx
-
Brevibacterium sterolicum
cholesterol oxidase
-
Streptomyces hygroscopicus
cholesterol oxidase
-
Brevibacterium sterolicum
ShChOx
-
Streptomyces hygroscopicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
0.8
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
1
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
3
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Streptomyces hygroscopicus
6
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Streptomyces hygroscopicus
6
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
7
-
trans-androsterone substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
8.2
-
trans-dehydroandrosterone substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
9
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
11
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
21
-
pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
21
-
pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
24
-
pregnenolone substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
32
-
cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
35
-
pregnenolone substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
37
-
cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
40
-
cholestanol substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
43
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
48
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
48
-
cholesterol substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
56
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum
57
-
cholesterol substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 Brevibacterium sterolicum
63
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 Streptomyces hygroscopicus
67
-
cholesterol substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 Brevibacterium sterolicum

Cofactor

Cofactor Comment Organism Structure
FAD covalently linked Brevibacterium sterolicum
FAD noncovalently bound to the enzyme Streptomyces hygroscopicus