KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
cholesterol | substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.07 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.11 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.14 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.17 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.2 | - |
cholestanol | substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.2 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.2 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.2 | - |
pregnenolone | substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.2 | - |
pregnenolone | substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.2 | - |
pregnenolone | substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.2 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.25 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.25 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.3 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.4 | - |
cholesterol | substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.4 | - |
pregnenolone | substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.5 | - |
trans-androsterone | substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.7 | - |
cholestanol | substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.8 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
0.8 | - |
trans-androsterone | substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.9 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
1.2 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
- |
- |
Streptomyces hygroscopicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholestanol + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
cholestanol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
cholesterol + O2 | - |
Streptomyces hygroscopicus | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
pregnenolone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
pregnenolone + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
trans-androsterone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
trans-androsterone + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
trans-dehydroandrosterone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
trans-dehydroandrosterone + O2 | - |
Brevibacterium sterolicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BsChOx | - |
Brevibacterium sterolicum |
cholesterol oxidase | - |
Streptomyces hygroscopicus |
cholesterol oxidase | - |
Brevibacterium sterolicum |
ShChOx | - |
Streptomyces hygroscopicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
trans-androsterone | substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
0.8 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
1 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
3 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
6 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
6 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
7 | - |
trans-androsterone | substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
8.2 | - |
trans-dehydroandrosterone | substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
9 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
11 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
21 | - |
pregnenolone | substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
21 | - |
pregnenolone | substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
24 | - |
pregnenolone | substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
32 | - |
cholesterol | substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
35 | - |
pregnenolone | substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
37 | - |
cholestanol | substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
40 | - |
cholestanol | substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
43 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Brevibacterium sterolicum | |
48 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
48 | - |
cholesterol | substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
56 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum | |
57 | - |
cholesterol | substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5 | Brevibacterium sterolicum | |
63 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5 | Streptomyces hygroscopicus | |
67 | - |
cholesterol | substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5 | Brevibacterium sterolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently linked | Brevibacterium sterolicum | |
FAD | noncovalently bound to the enzyme | Streptomyces hygroscopicus |