Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.7 extracted from

  • Ferreira, P.; Ruiz-Duenas, F.J.; Martinez, M.J.; van Berkel, W.J.; Martinez, A.T.
    Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme (2006), FEBS J., 273, 4878-4888.
    View publication on PubMed
Search for more literature for 1.1.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Emericella nidulans under control of the inducible fungal alc promoter Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
F501A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pleurotus eryngii
F501Y site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pleurotus eryngii
H502L site-directed mutagenesis, inactive mutant Pleurotus eryngii
H502R site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Pleurotus eryngii
H502S site-directed mutagenesis, inactive mutant Pleurotus eryngii
H546L site-directed mutagenesis, inactive mutant Pleurotus eryngii
H546R site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Pleurotus eryngii
H546S site-directed mutagenesis, inactive mutant Pleurotus eryngii
L315A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Pleurotus eryngii
Y78A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pleurotus eryngii
Y92A site-directed mutagenesis, inactive mutant Pleurotus eryngii
Y92F site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 4-anisyl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
0.026
-
 4-anisyl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
0.027
-
 4-anisyl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
0.039
-
 4-anisyl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
0.04
-
 4-anisyl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
0.053
-
 4-anisyl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
0.081
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
0.094
-
 2,4-hexadien-1-ol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
0.113
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
0.114
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
0.168
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
0.211
-
 3-anisyl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
0.215
-
 3-anisyl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
0.227
-
 3-anisyl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
0.263
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
0.293
-
 3-anisyl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
0.301
-
 3-anisyl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
0.317
-
 veratryl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
0.38
-
 veratryl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
0.46
-
 veratryl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
0.49 2 veratryl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
0.54
-
 veratryl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
0.614
-
 benzyl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
0.632
-
 benzyl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
0.639
-
 benzyl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
0.719
-
 benzyl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
0.734
-
 3-anisyl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
0.844
-
 veratryl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
0.985
-
 benzyl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
2.5 5 benzyl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, active site structure, Tyr92, Phe501, His502 and His546 are strictly required for activity, Tyr78 is not involved in catalysis, while Leu315 might be important Pleurotus eryngii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of wild-type an dmutant enzymes Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-hexadien-1-ol + O2
-
 Pleurotus eryngii 2,4-hexadienal + H2O2
-
 ?
2-naphthalenemethanol + O2
-
 Pleurotus eryngii ?
-
 ?
3-anisyl alcohol + O2
-
 Pleurotus eryngii 3-anisyl aldehyde + H2O2
-
 ?
4-anisyl alcohol + O2
-
 Pleurotus eryngii 4-anisyl aldehyde + H2O2
-
 ?
benzyl alcohol + O2
-
 Pleurotus eryngii benzaldehyde + H2O2
-
 ?
cinnamyl alcohol + O2
-
 Pleurotus eryngii cinnamyl aldehyde + H2O2
-
 ?
additional information an H2O2-producing ligninolytic enzyme, molecular docking study of substrates, overview Pleurotus eryngii ?
-
 ?
veratryl alcohol + O2
-
 Pleurotus eryngii veratryl aldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Pleurotus eryngii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 benzyl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
1
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
1
-
 3-anisyl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
3
-
 veratryl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
3
-
 4-anisyl alcohol pH 6.0, 24°C, mutant F501A Pleurotus eryngii
8
-
 3-anisyl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
12
-
 3-anisyl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
15
-
 3-anisyl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
17
-
 3-anisyl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
19
-
 benzyl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
25
-
 benzyl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
26
-
 3-anisyl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
27
-
 benzyl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
30
-
 benzyl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
33
-
 benzyl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
56
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
60
-
 4-anisyl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
76
-
 veratryl alcohol pH 6.0, 24°C, mutant L315A Pleurotus eryngii
83
-
 veratryl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
86
-
 veratryl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
90
-
 4-anisyl alcohol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
110
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
111
-
 4-anisyl alcohol pH 6.0, 24°C, mutant F501Y Pleurotus eryngii
114
-
 veratryl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
116
-
 veratryl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
119
-
 2,4-hexadien-1-ol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
139
-
 4-anisyl alcohol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii
142
-
 4-anisyl alcohol pH 6.0, 24°C, wild-type enzyme Pleurotus eryngii
177
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant Y78A Pleurotus eryngii
206
-
 2,4-hexadien-1-ol pH 6.0, 24°C, mutant Y92F Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
FAD required for activity Pleurotus eryngii