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Literature summary for 1.1.3.9 extracted from

  • Parikka, K.; Leppänen, A.; Pitkänen, L.; Reunanen, M.; Willför, S.; Tenkanen, M.
    Oxidation of polysaccharides by galactose oxidase (2010), J. Agric. Food Chem., 58, 262-271.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the inactive form ofGO can be oxidized to the active radical by peroxidases Fusarium sp.

Application

Application Comment Organism
synthesis galactose oxidase is used as a catalyst to oxidize selectively the C-6 hydroxyls of terminal galactose to carbonyl groups, e.g. polysaccharides studied included spruce galactoglucomannan, guar galactomannan, larch arabinogalactan, corn fiber arabinoxylan, and tamarind seed xyloglucan, with terminal galactose. A multienzyme system is used, with catalase and horseradish peroxidase to enhance the action of galactose oxidase, GC-MS analysis, overview Fusarium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ required, copper enzyme. The active site of the enzyme contains a tyrosine residue and a copper atom, and in the active form of galactose oxidase the tyrosine is in the radical form and the copper atom at oxidation state +2 Fusarium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
1 * 65000, SDS-PAGE Fusarium sp.

Organism

Organism UniProt Comment Textmining
Fusarium sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Fusarium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl alpha-D-galactopyranoside + O2
-
Fusarium sp. methyl alpha-D-galacto-hexodialdose + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Fusarium sp.