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Literature summary for 1.1.5.2 extracted from
- Quinoprotein D-glucose dehydrogenase of the Acinetobacter calcoaceticus respiratory chain: membrane-bound and soluble forms are different molecular species (1989), Biochemistry, 28, 6276-6280.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.074 | - |
phenazine methosulfate | membrane-bound enzyme form | Acinetobacter calcoaceticus |  |
| 1.9 | - |
phenazine methosulfate | soluble enzyme form | Acinetobacter calcoaceticus | |
| 4.2 | - |
D-glucose | - |
Acinetobacter calcoaceticus | |
| 24.5 | - |
D-glucose | soluble enzyme | Acinetobacter calcoaceticus | |
| 26.7 | - |
lactose | soluble enzyme | Acinetobacter calcoaceticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Acinetobacter calcoaceticus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, soluble enzyme SDS-PAGE | Acinetobacter calcoaceticus |
| 83000 | - |
x * 83000, soluble enzyme, SDS-PAGE | Acinetobacter calcoaceticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble enzyme and membrane-bound enzyme | Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 2,6-dichlorophenol-indophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + phenazine methosulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, soluble enzyme SDS-PAGE | Acinetobacter calcoaceticus |
| ? | x * 83000, soluble enzyme, SDS-PAGE | Acinetobacter calcoaceticus |
| dimer | - |
Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
soluble enzyme | Acinetobacter calcoaceticus |
| 8.5 | - |
membrane-bound enzyme | Acinetobacter calcoaceticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5: about 40% of maximal activity, pH 9.5: about 85% of maximal activity, membrane-bound enzyme, reaction with phenazine methosulfate, 2,6-dichlorophenol indophenol and pyrroloquinoline quinone | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | prosthetic group | Acinetobacter calcoaceticus | |
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