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Literature summary for 1.1.5.2 extracted from

  • Heuberger, E.H.M.L.; Poolman, B.
    A spectroscopic assay for the analysis of carbohydrate transport reactions (2000), Eur. J. Biochem., 267, 228-234.
    View publication on PubMed

Application

Application Comment Organism
analysis enzyme is used as coupling enzyme for monitoring carbohydrate-transport reactions, the method is particularly suited for determining transport reactions that are not coupled to any form of metabolic energy such as uniport reactions, or for characterizing mutant proteins with a defective energy-coupling mechanism or system with high-affinity constants for sugars Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
D-glucose
-
Acinetobacter calcoaceticus
0.66
-
lactose
-
Acinetobacter calcoaceticus
27
-
melibiose
-
Acinetobacter calcoaceticus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Acinetobacter calcoaceticus
-
-

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenol-indophenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
?
lactose + 2,6-dichlorophenol-indophenol
-
Acinetobacter calcoaceticus ?
-
?
melibiose + 2,6-dichlorophenol-indophenol
-
Acinetobacter calcoaceticus ?
-
?

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone prosthetic group Acinetobacter calcoaceticus