Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.2 extracted from

  • Okuda, J.; Sode, K.
    PQQ glucose dehydrogenase with novel electron transfer ability (2004), Biochem. Biophys. Res. Commun., 314, 793-797.
    View publication on PubMed

Application

Application Comment Organism
biotechnology engineering of the soluble enzyme GDH-B to enable the electron transfer to the electrode in absence of artificial electron mediator by mimicking the domain structure of the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni, which is composed of a PQQ-containing catalytic domain and a cytochrome c domain Acinetobacter calcoaceticus
diagnostics enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen Acinetobacter calcoaceticus

Cloned(Commentary)

Cloned (Comment) Organism
gene gdhB, expression of the enzyme GDH-B as fusion protein C-terminally fused to cytochrome c domain from Comamonas testosteroni in Escherichia coli DH5alpha Acinetobacter calcoaceticus

Protein Variants

Protein Variants Comment Organism
additional information the recombinant cytochrome c-fusion protein shows a highly increased sensitivity when immobilized to the electrode as D-glucose sensor compared to the wild-type enzyme, overview Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 D-glucose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus
15
-
maltose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus
19
-
lactose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Acinetobacter calcoaceticus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for structure stabilization of the holoform Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, recombinant cytochrome c-fusion protein, SDS-PAGE Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ubiquinone Acinetobacter calcoaceticus
-
D-glucono-1,5-lactone + ubiquinol
-
?
D-glucose + ubiquinone Acinetobacter calcoaceticus LMD79.41
-
D-glucono-1,5-lactone + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Acinetobacter calcoaceticus LMD79.41
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant cytochrome c fusion protein from Escherichia coli Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindolphenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
?
D-glucose + 2,6-dichlorophenolindolphenol
-
Acinetobacter calcoaceticus LMD79.41 D-glucono-1,5-lactone + ?
-
?
D-glucose + ubiquinone
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
?
D-glucose + ubiquinone
-
Acinetobacter calcoaceticus LMD79.41 D-glucono-1,5-lactone + ubiquinol
-
?
lactose + 2,6-dichlorophenolindolphenol
-
Acinetobacter calcoaceticus ?
-
?
lactose + 2,6-dichlorophenolindolphenol
-
Acinetobacter calcoaceticus LMD79.41 ?
-
?
maltose + 2,6-dichlorophenolindolphenol
-
Acinetobacter calcoaceticus ?
-
?
maltose + 2,6-dichlorophenolindolphenol
-
Acinetobacter calcoaceticus LMD79.41 ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, recombinant cytochrome c-fusion protein, SDS-PAGE Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
GDH-B
-
Acinetobacter calcoaceticus
PQQ glucose dehydrogenase
-
Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3178
-
D-glucose recombinant cytochrome c-fusion protein, pH 7.0 Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone i.e. PQQ, dependent on Acinetobacter calcoaceticus

pI Value

Organism Comment pI Value Maximum pI Value
Acinetobacter calcoaceticus calculated, recombinant cytochrome c-fusion protein 4.7 4.6
Acinetobacter calcoaceticus calculated, wild-type GDH-B 4.7 4.6