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Literature summary for 1.1.5.2 extracted from
- The metal ion in the active site of the membrane glucose dehydrogenase of Escherichia coli (2003), Biochim. Biophys. Acta, 1647, 200-205.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D354N | site-directed mutagenesis, 9% of wild-type activity, mutant enzyme can be reconstituted with PQQ and Ca2+, Sr2+, or Ba2+, but not with Mg2+, which functions as a competitive inhibitor, in contrary to the wild-type enzyme | Escherichia coli |
| D354N/N355D | site-directed mutagenesis, 10% of wild-type activity, mutant enzyme can be reconstituted with PQQ and Ca2+, Sr2+, or Ba2+, but not with Mg2+, which functions as a competitive inhibitor, in contrary to the wild-type enzyme | Escherichia coli |
| N355D | site-directed mutagenesis, 25% of wild-type activity, mutant enzyme can be reconstituted with PQQ and Ca2+, Sr2+, or Ba2+, but not with Mg2+, which functions as a competitive inhibitor, in contrary to the wild-type enzyme | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | competitive to Mg2+, wild-type enzyme | Escherichia coli |  |
| Ca2+ | competitive to Mg2+, wild-type enzyme | Escherichia coli | |
| Mg2+ | competitive to Ca2+, Sr2+, or Ba2+, mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
| Sr2+ | competitive to Mg2+, wild-type enzyme | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | metal binding kinetics, wild-type and mutant enzymes, Km for the different substrates of mutant enzymes with different metal ions bound, overview | Escherichia coli | |
| 2.8 | - |
D-glucose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 3.5 | - |
2-deoxy-D-glucose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 17 | - |
D-xylose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 17.5 | - |
D-galactose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 31 | - |
L-arabinose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 79 | - |
3-O-methyl-D-glucose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 116 | - |
D-mannose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
| 166 | - |
D-ribose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
| periplasm | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
| Ca2+ | mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
| Mg2+ | required, bound at the active site, cannot be substituted by Ca2+, Sr2+, or Ba2+ | Escherichia coli | |
| Sr2+ | mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ubiquinone | Escherichia coli | transfers electrons to the cytochrome oxidase through ubiquinone in the electron transport chain | D-glucono-1,5-lactone + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P15877 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol | active site structure | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + ubiquinone | - |
Escherichia coli | 2-deoxy-D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 3-O-methyl-D-glucose + ubiquinone | - |
Escherichia coli | 3-O-methyl-D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-galactose + ubiquinone | - |
Escherichia coli | D-galactono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | - |
Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-glucose + ubiquinone | transfers electrons to the cytochrome oxidase through ubiquinone in the electron transport chain | Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| D-mannose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
| D-ribose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
| D-xylose + ubiquinone | - |
Escherichia coli | D-xylono-1,5-lactone + ubiquinol | - |
? | |
| L-arabinose + ubiquinone | - |
Escherichia coli | L-arabino-1,5-lactone + ubiquinol | - |
? | |
| additional information | substrate specificity of recombinant wild-type and mutant enzymes | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH | - |
Escherichia coli |
| membrane glucose dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40.5 | - |
10 min, 50% inactivation of recombinant mutant D354N apoenzyme | Escherichia coli |
| 40.8 | - |
10 min, 50% inactivation of recombinant wild-type apoenzyme | Escherichia coli |
| 41 | - |
10 min, 50% inactivation of recombinant mutant D354N/N355D apoenzyme | Escherichia coli |
| 43.2 | - |
10 min, 50% inactivation of recombinant mutant N355D apoenzyme | Escherichia coli |
| 48.6 | - |
10 min, 50% inactivation of recombinant mutant D354N/N355D holoenzyme | Escherichia coli |
| 49 | - |
10 min, 50% inactivation of recombinant mutant D354N holoenzyme | Escherichia coli |
| 51 | - |
10 min, 50% inactivation of recombinant wild-type holoenzyme | Escherichia coli |
| 55 | - |
10 min, 50% inactivation of recombinant mutant N355D holoenzyme | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | i.e. PQQ, dependent on, Escherichia coli needs to be reconstituted with PQQ for activity | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibitory effects of the different metal ions on recombinant wild-type and mutant enzymes | Escherichia coli |
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